摘要
应用高压冷冻-冷冻置换技术(HPF-FS)制备了马铃薯Y病毒属的小西葫芦黄花叶病毒(ZYMV)和大豆花叶病毒(SMV)侵染寄主植物叶细胞的超薄切片样品,并与传统化学固定方法进行比较。透射电镜观察结果显示:病毒粒子和内含体的形态分布在两种方法处理的样品中无明显差异,但高压冷冻样品的细胞超微结构精细,细胞壁与细胞质之间边界清晰,质膜平滑而完整,细胞基质丰富;细胞核和叶绿体、线粒体等形态饱满,高尔基体潴泡结构及分泌小泡清晰,在ZYMV感染细胞的叶绿体中观察到囊泡结构。而化学处理的样品普遍存在细胞结构的收缩和变形,特别是质膜褶皱,与细胞壁分离,叶绿体呈梭形,片层结构发生改变,高尔基体潴泡结构及分泌小泡相当少见。实验结果有利于正确区别病毒所引起的细胞病理变化和化学处理而产生的人工假象。
The high pressure freezing and freeze substitution（ HPF-FS） were used for ultrastructural analysis of the Zucchini yellow mosaic virus（ ZYMV ）-infected plant leaves and the Soybean mosaic virus （ SMV ）-infected plant leaves,and then compared with conventional chemical fixation.The results showed that the distribution of the virus particles and the cylindrical inclusions has no significant difference between the two processing methods,but the ultrastructure of the host cells are quite different.The ultrastructure of HPF-FS samples is finer.The border between the cell wall and cytoplasm is clear,the plasma membrane is smooth and integrity.The nucleus,chloroplasts,mitochondria and other organelles＇ form are full.The Golgi retention bubble and secretory vesicles are clear.And a vesicle structure is observed in some ZYMV-infected chloroplasts.But the cell structure of the chemical processed sample generally has contraction and deformation,especially the plasma membrane folds,the membrane separated from the cell wall.And the spindle-shaped chloroplasts have the changed lamellar structure.The Golgi retention bubble and secretory vesicles are very rare.All these help to correctly distinguish the pathological changes caused by plant viruses and the artificial feints due to chemical processing.
出处
《电子显微学报》
CAS
CSCD
北大核心
2010年第2期146-151,共6页
Journal of Chinese Electron Microscopy Society
基金
国家"863"高新技术发展计划资助(2008AA10Z129)
浙江省自然科学基金资助(Y307466)
关键词
高压冷冻-冷冻置换
小西葫芦黄花叶病毒
大豆花叶病毒
超微结构
high pressure freezing and freeze substitution
Zucchini yellow mosaic virus
Soybean mosaic virus
ultrastructure
