摘要
高压冷冻技术(high pressure freezing,HPF)是近年来出现的一种可以更有效保存样品原始结构的制样方法。本文介绍了针对高压冷冻制样过程中,无法区分用于培养细胞的蓝宝石盘正反两面的问题,使用3D打印技术制作模具进行喷涂特殊样式的碳膜标记,从而得以区分蓝宝石盘正反面,提高制样成功率。
High pressure freezing can efficiently preserve natural cellular ultra-structure.During the sample preparation process,it is hard to distinguish the side with cell of the sapphire disc.To resolve the problem,we developed an auxiliary tool by using a3D printing to lay a carbon film with specific pattern on the sapphire disc,which could mark the right side of sapphire disc.Several results have proved our tool could effectively improve the sample preparation quality
作者
贾旭东
李红梅
李茵茵
陈东
张勤奋
JIA Xu-dong;LI Hong-mei;LI Yin-yin;CHEN dong;ZHANG Qin-fen(School of Life Sciences Sun Yat-Sen University, Guangzhou Guangdong 510275,China;State Key Laboratory for Biocontrol,Sun Yat-Sen University, Guangzhou Guangdong 510275,China)
出处
《电子显微学报》
CAS
CSCD
2017年第5期514-516,共3页
Journal of Chinese Electron Microscopy Society
基金
国家自然科学基金资助项目(No.31570736
No.J1310025)
NSFC-RGC资助项目(No.31361163001)
关键词
高压冷冻
辅助装置
high pressure freezing
auxiliary tool
