摘要
利用Ｆｅ２＋－Ｃｙｓ－Ｈｉｓ体系诱发鼠肝线粒体和红细胞产生脂质过氧化的方法，研究了枸杞总黄酮类化合物（ＴＦＬ）的抗脂质过氧化作用。结果表明，ＴＦＬ可阻断该体系诱发的鼠肝线粒体脂质过氧化，当ＴＦＬ终浓度在０．０２５～２．０ｍｇ／ｍｌ范围时，对丙二醛（ＭＤＡ）生成阻断率为３８．３８％～９９．９６％，呈剂量－效应关系，同时具有保持线粒体膜流动性的作用。扫描电镜观察到ＴＦＬ对脂质过氧化所致红细胞形态结构的破坏有保护作用。
The protective effects of total flavonoids from Lycium Barbarum L.(TFL) on lipid peroxidation in mitochondria and red blood cells (RBC) induced by oxygen radicals produced by Fe 2+ cysteine system were investigated. The mitochondria lipid peroxidation (measured as malondialdehyde, MDA) was significantly inhibited by TFL with a dose response relation between the concentrations of 0 025 and 2 0 mg/ml, and the fluidity of mitochondria membrane was also protected effectively. It was observed by scan electron microscope, that the shape of RBC in the Fe 2+ system was damaged significantly. The shape of RBC was remained with the addition of TFL.
出处
《卫生研究》
CAS
CSCD
北大核心
1999年第2期115-116,共2页
Journal of Hygiene Research
基金
国家自然科学基金
关键词
枸札总黄酮
线粒体
脂质过氧化
实验研究
flavonoids of Lycium Barbarum L., lipid peroxidation, mitochondria
