摘要
目的 观察髓样分化因子88（MyD88）沉默的半成熟树突状细胞（DC）诱导大鼠小肠移植模型免疫耐受.方法 供体乃44大鼠,受体Wistar大鼠.随机分为3组（n=6）.A组（半成熟DC组）移植前7d经阴茎背静脉注射半成熟MyD88沉默DC（2×106细胞数）,B组（未成熟DC组）移植前7 d经阴茎背静脉注射未成熟DC（2×106细胞数）和C组（阴性对照组）移植前7 d经阴茎背静脉注射1 ml生理盐水.3组均于注射7 d后行大鼠异位节段性小肠移植术,观察统计各组受体存活情况,检测受体血清中的促炎症因子白细胞介素（IL）-2、干扰素（IFN）-γ等的变化.结果 半成熟MyD88沉默DC组[（13.7±1.2）d,n=6]较未成熟DC组[（8.0±1.0）d,n=6,P〈0.05]和阴性对照组[（6.0±0.8）d,n=6,P〈0.05）存活时间显著延长,同时受体血清中IL-2和IFN-γ等促炎症因子的表达明显降低（P〈0.05）,病理改变亦较轻微.结论 MyD88沉默的半成熟DC具有独特的表形和功能,能够诱导受体产生特异性免疫耐受,显著延长受体术后的存活时间.
Objective To study MyD88 silent semi-mature DC （simDC） inducing immune tolerance in rat small bowel transplantation model. Methods All rats were randomly divided into three groups （n=6）. Seven days before transplantation of intestine from F344 donors, Wistar recipient rats were injected with simDC （group A） , imDC （group B） and saline （ group C） through the penile dorsal vein respectively.Small bowel transplantation was performed and the survival time of recipients was observed. Serum IL-2 and IFN-γ levels were assayed. Results The survival time of recipients in group A was （ 13.7±1.2） days, which was significantly longer than that in groups B [（8.0±1.0） days,P〈0. 05] and C [（6. 0±0. 8） days P〈0. 05]. The serum levels of IL-2 and IFN-γ in group A were lower than in groups B and C （P〈0. 05）. Conclusion Depending on the unique phenotypic and functional features of sem-DC,they can induce the transplantation tolerance and prolong the survival of intestinal allografts after transplantatin.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2010年第6期760-762,I0002,共4页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（30872532）
关键词
树突状细胞
免疫耐受
MYD88
小肠移植
Dendritic cell
Immune tolerance
MyD88
Intestinal transplantation
