摘要
目的 探讨免疫耐受在移植领域的应用前景 ,建立猪节段性小肠移植免疫耐受模型。方法 12只幼猪为受者 ,随机分为实验组及对照组 ,另选体重相近的异性猪为供者 ,实验组用供者骨髓细胞预处理 ,2周后行节段性小肠移植 ,术后不使用免疫抑制剂 ;对照组不作预处理。结果 实验组部分移植小肠获长期存活 ,T细胞亚群明显下降 ,外周血白细胞介素 (IL) 2和IL 6均无明显变化 ,外周血白细胞发现嵌合现象 ,病理表现为轻度急性排异和慢性排异或无排异 ;对照组移植小肠存活时间多在 2周内 (5 / 6 ) ,T细胞亚群明显升高 ,外周血IL 2和IL 6均明显升高 ,外周血白细胞未发现嵌合现象 ,病理表现为重度急性排异。结论 猪供者骨髓细胞预处理受者能诱导供者特异性小肠移植免疫耐受 ,使移植物获长期存活 ,但这种免疫耐受是不完全的或不稳定的。
Objective\ To develop the model of immune tolerance in porcine partial small bowel transplantation. Methods\ As recipients, 12 young pigs were randomly divided into experimental group and control group. Other opposite sex pigs with near weight were used as donors, the experimental group was preconditioned with donor's bone marrow cells and partial small bowel transplantation was performed 2 weeks later without further immunosuppression; the control group was not preconditioned. Results\ In the experimental group, long-term graft survival was observed partially, T cell subgroups was descended obviously, IL-2 and IL-6 had no obvious change in peripheral blood, chimerism developed in peripheral white blood cells and pathological changes contained mild acute rejection and chronic rejection or no rejection. In control group, most graft survived for 2 weeks (5/6), T cell subgroups was increased obviously, IL-2 and IL-6 markedly elevated in peripheral blood, no chimerism developed in peripheral white blood cells and pathological changes included severe acute rejection in all. Conclusion\ The donor-specific immune tolerance of small bowel transplantation in pigs could be induced by preconditioning of the recipient with donor's bone marrow cells, but this tolerance was not complete or stable.\;
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2000年第4期308-310,共3页
Chinese Journal of Experimental Surgery
关键词
小肠移植
免疫耐受
移植物排斥
猪
Small bowel/transplantation
\ Immune tolerence
\ Graft rejection
