摘要
目的观察ApoE基因敲除（ApoE^-/-）小鼠的骨微结构、骨密度、骨矿含量（BMC）的变化，探讨ApoE在骨重建中的作用。方法15、28和40周龄雄性ApoE^-/-纯合子小鼠以及同性别、同周龄的野生型小鼠共48只，应用显微CT测定小鼠右侧股骨远端松质骨和皮质骨的骨微结构参数，双能X线骨密度仪（DXA）测定左侧股骨骨密度。并分析骨微结构、骨密度、BMC相关性。结果与野生鼠相比，不同时期ApoE^-/-鼠的股骨松质骨体积骨密度（vBMD）、组织骨密度、BMC、骨体积分数（BV／TV）、骨小梁数量（Tb．N）、骨小梁厚度（Tb．Th）明显增加（P〈0．05），骨面积分数（BS／BV）、骨小梁间隔（Tb．SP）和结构模型指数明显减低（P〈0．05）。股骨皮质骨内径周长、皮质骨外径周长、皮质骨面积、骨髓腔面积、截面总面积和截面惯性矩增加，而皮质骨骨密度、皮质骨BMC和皮质骨厚度变化不明显。DXA测定显示28周龄ApoE^-/-鼠的总体骨密度明显高于野生型鼠（P〈0．05），15和40周龄ApoE“鼠总体骨密度与对照组相比，无统计学差异。28周龄ApoE^-/-鼠vBMD与BMC、BV／TV、Tb．Th、BS／BV和皮质骨BMC明显相关，相关系数分别为0．955、0．944、0．834、0．923和0．903，而与其他参数不相关。结论ApoE^-/-鼠表现出骨量增加，提示ApoE在骨重建中起重要作用。
Objective To study the changes of microarchitecture, bone mineral density （BMD）, and bone mineral content （BMC） in apolipoprtein E knockout （ApoE^-/-） mice. Methods Male ApoE^-/- mice at 15, 28, and 40-week of age and sex-age-matched wild-type （WT） mice were involved in the study. The trabecular and cortical bone microarchitecture were assessed by micro-CT（μCT）in the right distal femur. The total body BMD of the left femur was determined by dual-energy X-ray absorptiometry （DXA）. The relationships among BMD, microarchitecture, and BMC were analyzed. Results Compared with WT mice, the advancing age ApoE^-/- mice showed an increased volumetric BMD （vBMD）, tissue BMD （tBMD）, BMC,bone volume fraction （BV/TV）, trabecular number （Tb. N）, trabecular thickness （ Tb. Th） with an decreased bone surface fraction （ BS/BV）, trabecular separation （Tb. SP）, and the structure mode index（P〈0.05）in the cancellous bone of femur. The cortical bone microarchitecture parameters as inner perimeter, outer perimeter, cortical area, marrow area, total area and moment of inertia were also increased, but cortical BMD, cortical bone mineral content （C. BMC） and cortical thickness retained constant. At the age of 28 weeks, the total body BMD in ApoE^-/- mice revealed higher than WT mice （P〈0.05） and there was no changes in 15 and 40-week-old mice compared with the sex-age-matched controls, vBMD was positively correlated with BMC, BV/TV,Tb. Th, BS/BV, and C. BMC, with the correlation coefficients 0.955,0. 944,0. 834,0.923, and 0. 903, respectively, and there was no correlation between vBMD and the other parameters. Conclusions ApoE^-/- mice display an increased bone mass, suggesting that ApoE has an important role in bone remodeling.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2010年第5期406-410,共5页
Chinese Journal of Endocrinology and Metabolism
基金
国家自然科学基金资助项目（3700891）
关键词
载脂蛋白E
基因敲除
骨密度
骨微结构
Apolipoprotein E
Gene knockout
Bone mineral density
Bone microarchitecture
