摘要
目的观察ω-3多不饱和脂肪酸(PUFA)对去势大鼠骨微结构的影响。方法将30只雌性大鼠随机分为假手术组、模型组和实验组。假手术组仅切除卵巢周围脂肪组织,其余两组切除双侧卵巢。模型组和假手术组给予1 m L/(100 g·d)的生理盐水灌胃,实验组给予ω-3 PUFA 150 mg/(kg·d)。干预12 w后,处死所有大鼠,检测血钙、磷、碱性磷酸酶(ALP)和雌激素水平。分离右侧整段股骨应用micro-CT检测骨密度和骨微结构。结果雌二醇在3组之间存在显著差异,表现为模型组水平最低(8.1±2.8)mmol/L,而假手术组水平最高(25.0±8.9)mmol/L(P<0.05)。实验组松质骨骨密度[(0.180±0.022)g/cm3vs(0.135±0.017)g/cm3]、BV/TV[(36.422±10.893)%vs(29.631±9.705)%]、Tb.Th[(0.138±0.044)mm vs(0.117±0.039)mm]和Tb.N[(2.252±0.078)/mm vs(1.589±0.083)/mm]均显著高于模型组,而Tb.Sp[(0.404±0.020)mm vs(0.599±0.032)mm]显著低于模型组(P<0.05);实验组皮质骨骨密度[(0.718±0.015)g/cm3vs(0.633±0.025)g/cm3]、BV/TV[(48.559±7.152)%vs(33.878±8.764)%]和Cr.Th[(0.483±0.037)mm vs(0.428±0.034)mm]均显著高于模型组,而孔隙率[(32.162±7.891)%vs(38.859±9.847)%]显著低于模型组(P<0.05)。结论ω-3PUFA对去卵巢大鼠的骨微结构有一定的改善作用,这对于防治骨质疏松具有一定的意义。
Objective To observe the effect of ω-3 polyunsaturated fatty acids（ PUFA） on bone micro-architecture in ovariectomized rats. Methods Thirty female rats were randomly divided into sham operation group, model group, and experimental group. The ovary and the surrounding adipose tissue were removed in rats of sham group,and the rats in remaining two groups were ovariectomized. Rats in model group and sham group received normal saline of 1ml /100 g / d and rats in experimental group received ω-3 PUFA of 150 mg / kg / d. After 12 weeks,all the rats were sacrificed. Serum calcium,phosphorus,alkaline phosphatase（ ALP）,and estradiol were detected. The entire right femurs were collected and bone mineral density and microstructure were detected with micro-CT. Results Estradiol level was significantly different among the three groups,with the lowest level in model group（ 8. 1 ± 2. 8 mmol / L） and the highest level in sham group（ 25. 0 ± 8. 9 mmol / L,P 〈 0. 05）. Trabecular bone mineral density（ 0. 180 ± 0. 022 g / cm3 vs 0. 135 ± 0. 017 g / cm3）,BV / TV（ 36. 422 ± 10. 893% vs 29. 631 ± 9. 705%）,Tb. Th（ 0. 138 ± 0. 044 mm vs 0. 117 ± 0. 039 mm） and Tb. N（ 2. 252 ± 0. 078 / mm vs 1. 589 ± 0. 083 / mm） in the experimental group were significantly higher than those in the model group,but Tb. Sp（ 0. 404 ± 0. 020 mm vs 0. 599 ± 0. 032 mm） was significantly lower than that in the model group（ P 〈 0. 05）. Cortical bone mineral density（ 0. 718 ± 0. 015 g / cm3 vs 0. 633 ± 0. 025 g / cm3）,BV /TV（ 48. 559 ± 7. 152% vs 33. 878 ± 8. 764%） and Cr. Th（ 0. 483 ± 0. 037 mm vs 0. 428 ± 0. 034 mm） in the experimental group were significantly higher than those in the model group,but the porosity（ 32. 162 ± 7. 891% vs 38. 859 ±9. 847%） was significantly lower than that in the model group（ P 〈 0. 05）. Conclusion ω-3 PUFA improves bone microstructure in ovariectomized rats to some extent,which is of significance for prevention and treatment of osteoporosis.
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2017年第1期31-34,共4页
Chinese Journal of Osteoporosis
关键词
Ω-3多不饱和脂肪酸
骨质疏松
骨微结构
ω-3 Polyunsaturated fatty acids
Osteoporosis
Bone micro-architecture
