摘要
目的观察护肝解纤汤对肝纤维化大鼠肝功能、肝纤维化指标的影响,探讨其对肝纤维化的治疗作用。方法以四氯化碳腹腔内注射的方法建立大鼠肝纤维化模型,造模后施以由低、中、高剂量姜黄及其他中药组成的护肝解纤汤,以小柴胡汤和复方鳖甲软肝片为阳性对照。12周后最大量取血并处死大鼠。检测血清肝功能、Ⅲ型前胶原(PC-Ⅲ)、透明质酸(HA)、层粘连蛋白(LN)含量。结果模型组大鼠血清中ALT、AST含量升高,而ALB含量有所降低,各药物处理组ALT、AST较模型组降低,ALB较模型组升高,以复方鳖甲软肝片组更明显(P<0.05)。模型组大鼠肝纤维化指标较正常组明显升高,各药物处理组纤维化指标均较模型组降低,尤以低姜黄组和复方鳖甲软肝片组降低明显(P<0.05)。结论护肝解纤汤可改善肝纤维化大鼠肝功能,显著降低肝纤维化指标,具有治疗肝纤维化的作用。
Objective To observe effects of Huganjiexian decoction on changes of liver function and indexes of hepatic fibrosis of rat hepatic fibrosis,and to discuss therapeutic effects of Huganjiexian decoction on hepatic fibrosis.Methods Rat models of hepatic fibrosis were constructed by intraperitoneal injection of tetrachloride.Huganjiexian decoction composed of low,middle,high dose curcumin were given to these rats respectively at the same time.Sho-saiko-to compound treatment group and Fufangbiejiarangan tablets treatment group were made as positive groups.After twelve weeks,all rats were executed.Serum samples were kept to measure serum levels of ALT,AST,ALB,PC-Ⅲ,LN and HA.Results Serum levels of ALT and AST increased,and the level of ALB decreased in fibrotic group.In decoction groups,the levels of ALT and AST were lower than those in fibrotic group while the level of ALB was higher than that in fibrotic group,especially in Fufangbiejiarangan tablets group（P0.05）.Serum levels of PC-Ⅲ,LN,HA decreased obviously in decoction groups compared with fibrotic group,especially in the low-dose curcumin group and Fufangbiejiarangan tablets group（P0.05）.Conclusion Huganjiexian decoction can improve liver function and induce the serum index of hepatic fibrosis significantly,and it shows therapeutic effects on hepatic fibrosis.
出处
《胃肠病学和肝病学杂志》
CAS
2010年第5期420-422,共3页
Chinese Journal of Gastroenterology and Hepatology
基金
广东省科学技术厅课题(2008B030301011)
广东省中医药局科研课题(2008003)
广州市医药卫生科技项目(2008A30)
关键词
肝纤维化
护肝解纤汤
肝功能
Hepatic fibrosis
Huganjiexian decoction
Liver function
