摘要
目的检测肝纤维化大鼠血清及肝组织中基质金属蛋白酶-1(MMP-1)和基质金属蛋白酶组织抑制因子-1(TIMP-1)表达,探讨MMP-1和TIMP-1在肝纤维化中的作用。方法 20只雄性SD大鼠随机均分正常组及模型组,每组各10只,其中模型组以四氯化碳腹腔注射方法制作大鼠肝纤维化模型,12周后同时处死大鼠,用酶联免疫吸附测定法、免疫组化染色检测血清MMP-1及TIMP-1含量及肝组织MMP-1及TIMP-1表达的变化,两组间数据采用统计软件SPSS 12.0进行分析。结果①与正常组(553.81±98.89)μg/L比较,模型组血清MMP-1含量较正常组明显下降(t=13.38,P<0.01);模型组TIMP-1含量较正常组(10.17±1.35)μg/L升高,差异均有高度统计学意义(t=31.92,P<0.01)。②模型组肝组织MMP-1免疫组化图像分析面积百分比为(5.60±1.51)%,较正常组(19.20±1.48)%明显下降(t=14.37,P<0.01);模型组TIMP-1免疫组化图像分析面积百分比较正常组明显升高,差异均有高度统计学意义(t=38.96,P<0.01)。结论模型组血清及肝组织MMP-1子IMP-1下降下表达减少,而TIMP-1表达增加,TIMP-1表达增加可能在肝脏纤维化过程发挥重要作用。
Objective To detect the expression of matrix metalloproteinase-1（MMP-1） and tissue inhibitor of metallo proteinase-1（TIMP-1） of hepatic fibrosis rats and discuss its role in hepatic fibrosis.Methods 20 male SD rats were divided into normal group and model group randomly with 10 rats in each group： model group were induced by in traperitional injection of carbon tetrachloride.All rats were sacrificed at the same time after 12 weeks.The levels of MMP-1 and TIMP-1 were detected in serum and liver tissue by ELISA and immunohistochemical staining respective ly,the data was analyzed by statistical software of SPSS 12.0.Results ①Compared with the normal group（553.81 ± 98.89） μg/L,serum levels of MMP-1 in model group was found decreased（t = 13.38,P 0.01）,TIMP-1 in model group was found increased,the differences were statistically significant（t = 31.92,P 0.01）.② Hepatic tissue immunohistochemical image analysis area percentage of MMP-1 in model group was（5.60 ±1.51）%,it was lower than that in normal group [（19.20±1.48）%]（t = 14.37,P 0.01）;hepatic tissue immunohistochemical image analysis area per centage of TIMP-1 in model group was higher than that in normal group,the differences were statistically significant（t = 38.96,P 0.01）.Conclusion The levels of MMP-1 decreases and TIMP-1 increases significantly in liver tissues in model group,TIMP-1 may play a important role in hepatic fibrosis.
出处
《中国医药导报》
CAS
2013年第23期22-23,27,共3页
China Medical Herald
基金
广东省广州市中医药和中西结合科研项目立项资助项目(编号20112A011014)
