摘要
目的研究奥曲肽(OCT)对体外培养的大鼠肝星状细胞(HSC)胶原分泌及TIMP-1mRNA、MMP-2mRNA表达的影响。方法体外培养大鼠HSC,HSC随机分为4组,分别加入不同浓度的OCT,用ELISA法检测细胞上清液中的Ⅰ、Ⅲ型胶原的含量,半定量RT-PCR法检测OCT对HSC中MMP-2、TIMP-1 mRNA表达的影响。结果应用OCT干预后,HSC合成Ⅰ、Ⅲ型胶原减少,TIMP-1 mRNA的表达较少,而MMP-2 mRNA表达明显增加,差异有显著性(P<0.05)。结论OCT可以抑制HSC的Ⅰ、Ⅲ型胶原的合成,增强MMP-2mRNA的表达,抑制TIMP-1mRNA的表达,这可能是OCT发挥抗肝纤维化作用的途径之一。
Objective To investigate the effect of octreotide on the expression of collagen,TIMP - 1 and MMP -2 in cultured rat hepatic stellate cell （HSC） in vitro. Methods HSC cultured in vitro were randomly divided into 4 groups and different concentrations of octreotide were added to the cell groups. The synthesis of collagen type Ⅰ and type Ⅲ was meas- tired by enzyme - linked immunoaddsordent assay （ ELISA）, the expression of TIMP - 1 and MMP - 2 was detected by semi - quantitative RT - PCR. Results Octreotide remarkably reduced the production of collagen type Ⅰ and type Ⅲ. Oc- treotide down - regulated the expression of TIMP - 1 mRNA, while up - regulated the expression of MMP - 2 mRNA. （all P 〈0. 05）. Conclusion Octreotide could significantly reduced the content of collagen type I and type m. Octreotide can promot the expression of TIMP- 1 mRNA, while inhibite the expression of MMP-2 mRNA. All of these biological effects of octreotide on HSC were associated with recovery from liver fibrosis.
出处
《临床肝胆病杂志》
CAS
2008年第5期332-334,共3页
Journal of Clinical Hepatology
基金
基金项目:福建省科技厅(9911)
