摘要
进行了兽疫链球菌NW-162高密度发酵HA的工艺研究,通过发酵过程中不同饱和度的溶解氧和不同pH调控方法对该菌株发酵生产透明质酸的影响进行了研究。实验表明,采用30%饱和度的溶解氧和在发酵过程的0~30 h,调pH 7.2,发酵后期调pH 6.8的调控方法,OD660可达0.84,透明质酸产量为0.532 g/L。在此条件下,有利于细胞生长和产物表达,降低乙酸等有害代谢副产物积累。
The high cell-density fermentation process for producing hyaluronic acid with a streptococcus zooepidemius mutant NW-162 was studied.The different dissolved oxygen（DO） and different pH was optimized during fermentation process.The result showed the maximal yield of hyaluronic acid was 0.532 g/L and DO660 value reached 0.84,when the dissolved oxygen was controlled at 30% and pH value was at pre-term and mid-term pH 7.2,final-term pH 6.8 during different terms of fermentation.Under the conditions,it was beneficial to the growth of cell and aim product expression and it could reduce the accumulation of acetic acid.
出处
《应用化工》
CAS
CSCD
2010年第5期655-657,共3页
Applied Chemical Industry
基金
陕西省教育厅科研基金资助项目(01JK77)
关键词
兽疫链球菌
高密度发酵
透明质酸
streptococcus zooepidemics
high-cell-density fermentation
hyaluronic acid
