摘要
以‘小偃54’、‘京411’等67份小麦品种(系)为试材,对强光诱导小麦叶片花青素积累的现象及品种间变异进行分析,以探讨花青素在小麦响应强光过程中的生理与分子机制。结果显示,‘小偃54’和‘京411’分别经25℃强光处理24 h和48 h叶片花青素含量大幅增加,而15℃和30℃强光处理增幅较小;强光处理48 h后,‘京411’的花青素含量高达7.2μg.g-1FW,约为‘小偃54’的10倍。对‘京411’而言,花青素含量随叶位自下而上递减,并且按叶鞘、叶基部、叶中部和叶尖的部位依次递减。强光处理24 h和48 h后能诱导‘小偃54’和‘京411’的PAL活性增强。强光处理48 h后,紫色胚芽鞘类品种的花青素含量高于绿色胚芽鞘类品种,不同品种强光下的花青素吸收峰均在520 nm处。研究表明,强光下叶片花青素含量升高可能是紫色胚芽鞘类小麦品种抗强光的一种机制。
In order to explore high light induced anthocyanin accumulation in wheat（Triticum aestivum） seedlings,two winter wheat varieties（＇Xiaoyan 54＇ and ＇Jing 411＇） and other 65 varieties at three-leaf stage were measured for anthocyanin content when subjected to high light.The results showed that the anthocyanin contents in leaves of ＇Xiaoyan 54＇ and ＇Jing 411＇ increased greatly when subjected to high light for 24 and 48 h at 25℃ while increased slowly at both 30℃ and 15℃.At 25℃when ＇Jing 411＇ was subjected to high light for 48 h,its anthocyanin content was 7.2 μg·g-1FW,about ten times higher than that of ＇Xiaoyan 54＇.For ＇Jing 411＇ treated under high light for 48 h,its anthocyanin content decreased from first to third leaf as well as according to leaf sheath,leaf base,middle leaf part,and leaf tip.In agreement with the increase of anthocyanin content by high light,phenylalanine ammonia-lyase activity was also induced by high light in both varieties.Further,high light induced anthocyanin accumulation was dependent upon genotypes.We found that varieties with purple coleoptiles accumulated more anthocyanin than those with green coleoptiles.Additionally,the Absorbance peak of anthocyanin extracts in different varieties was at 520 nm.The high light induced anthocyanin accumulation may be a mechanism for purple coleoptiles varieties to cope with high light stress.This study might benefit for further study on the role of anthocyanin for wheat to conquer high light.
出处
《西北植物学报》
CAS
CSCD
北大核心
2010年第4期754-761,共8页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金项目(30800683)
国家重点基础研究计划(2002CB111304,2009CB118506)
关键词
小麦
花青素
强光
品种
温度
变异
wheat
anthocyanin
high light
variety
temperature
variation
