摘要
以薄皮木组培苗为试材,采用不同浓度(50、100、150、200、250 mmol·L^(-1))盐碱胁迫,以不加盐碱溶液为对照,测定薄皮木叶片丙二醛(MDA)、过氧化物酶(POD)、过氧化氢酶(CAT)及叶绿素含量的动态变化,研究盐碱胁迫对薄皮木幼苗生理特性的影响,确定薄皮木幼苗耐盐碱能力,以期为其引种栽培提供参考依据。结果表明:随着盐碱胁迫浓度的增加和胁迫时间的延长,丙二醛含量变化呈上升趋势;随着盐碱浓度的增加和胁迫时间的延长,CAT活性均呈先上升后下降趋势;随着盐浓度的增加和胁迫时间的延长,POD活性呈先升后降趋势,盐胁迫峰值为50 mmol·L^(-1),随着碱浓度的增加和胁迫时间的延长,POD活性变化不一致;随着盐碱胁迫浓度的增加和胁迫时间的延长,叶绿素含量呈下降趋势,碱胁迫下,50、100 mmol·L^(-1)与对照无显著差异,说明薄皮木幼苗具有一定的耐盐碱能力。在盐碱浓度50 mmol·L^(-1)时,可以通过自身生理生化变化来保护植株对环境的适应性,在盐碱浓度大于150 mmol·L^(-1)时,薄皮木幼苗会受到伤害,影响植株正常生长过程。
The tissue culture plantlets of Leptodermis oblonga were used as the test materials,and different concentrations(50,100,150,200,250 mmol·L^(-1))of saline-alkali stress were used,and the no saline-alkali solution was used as the control,malondialdehyde(MDA)content,peroxidase(POD)activity,catalase(CAT)activity and chlorophyll content in the leaves of Leptodermis oblonga were determined to study the effects of saline-alkali stress on physiological characteristics of Leptodermis oblonga seedlings,the salt-alkali tolerance of the seedlings was determined,in order to provide reference for its introduction and cultivation.The results showed that MDA content increased with the increase of saline-alkali stress concentration and the extension of stress time,with the increase of saline-alkali stress concentration and the extension of stress time,the activity of CAT increased first and then decreased.With the increase of salt stress concentration and the extension of stress time,the POD activity increased first and then decreased,and the peak value of salt stress was 50 mmol·L^(-1),the change of POD activity was not consistent under alkali stress.With the increase of salt stress concentration and the extension of stress time,the content of chlorophyll decreased,there was no significant difference between 50 mmol·L^(-1),100 mmol·L^(-1) and the control under alkali stress,which indicated that Leptodermis oblonga seedlings had a certain ability of salt tolerance.At a salinity concentration of 50 mmol·L^(-1),the plant adaptation to the environment could be protected by its own physiological and biochemical changes.When the salinity concentration was greater than 150 mmol·L^(-1),the seedlings of Leptodermis oblonga were damaged by salinity stress,and affected the normal process of plant growth.
作者
郭丽
朱飞雪
柴梦颖
李庆伟
韩春叶
张忠强
GUO Li;ZHU Feixue;CHAI Mengying;LI Qingwei;HAN Chunye;ZHANG Zhongqiang(Modern Horticultural College,Henan Vocational College of Agriculture,Zhengzhou,Henan 451450)
出处
《北方园艺》
CAS
北大核心
2024年第19期45-51,共7页
Northern Horticulture
基金
河南省科技攻关资助项目(232102110183)
河南农业职业学院特色花卉植物资源开发与应用科研创新团队资助项目(ZZ2405TD01)
河南农业职业学院花卉生产与应用科研创新团队资助项目(HNACKT-2023-03)。
关键词
薄皮木
幼苗
盐碱胁迫
生理生化指标
Leptodermis oblonga Bunge.
seedlings
saline-alkali stress
physiological and biochemical index
