摘要
目的研究虎杖中白藜芦醇的最佳提取工艺。方法采用L9(34)正交实验,以白黎芦醇的提取率为评价指标。结果实验设计因素中酶解加水量、酶解时间和提取溶剂用量对白藜芦醇的提取率有显著影响。结论酶解法提取虎杖中白藜芦醇的最佳条件为:纤维素酶用量20mg/g,酶解加水量3倍,酶解时间6d,提取溶剂用量9倍。酶解法提高了虎杖中白藜芦醇的提取率。
Objective：To investigate the best extraction turn Sieb et Zucc. Methods：A four-factor-three-level o process for resveratrol from Polygonum cuspidarthogonal array design was developed for the optimization of the extraction using the resveratrol yield as indexes. Result： Signficant effects were amount of water, enzymolysis time and amount of extracting solvent . Concision：The optimum conditions for enzymolysis were cellulase dosage 20mg/g , 3 times of water,6 days for enzymolysis and 9 times of methanol. The enzymolysis method can increase the extraction yield of resveratrol from Polygonum cuspidatum.
出处
《贵州师范大学学报（自然科学版）》
CAS
2010年第2期104-106,共3页
Journal of Guizhou Normal University：Natural Sciences
基金
贵州省科技计划项目(黔科合GY字[2008]3026)
贵阳市科学技术计划项目([2008]筑科农合同字第25号
贵阳市科学技术计划项目[2009]筑科工合同字第1-069号
贵州省中药材现代产业技术体系专项经费资助(黔农计[2009]236号)
关键词
虎杖
纤维素酶酶解
白藜芦醇
正交试验
Polygonum cuspidatitm
cellulase degradation
resveratrol
orthogonal design
