摘要
目的:研究左归丸提取液对乙型肝炎病毒e抗原(HBeAg)、表面抗原(HBsAg)的抑制作用。方法:应用2.2.15细胞株作为体外抗乙型肝炎病毒(HBV)实验模型,通过细胞毒性实验以确定对细胞无毒性作用的最高药物浓度,将此浓度药物直接加到细胞培养液中,分别取第4天、第8天的细胞培养上清液,用固相放射免疫方法测定其HBsAg、HBeAg含量。结果:浓度为25、12.5、6.25、3.125mg/ml的左归丸提取液对HBsAg、HBeAg有明显抑制作用;各浓度间存在量效关系;对HBeAg的作用优于对HBsAg的作用。结论:左归丸提取液对2.2.15细胞HBV标志物有显著的抑制作用。
Objective：To study the inhibition effect of Zuoguiwan extraction to HBeAg and HBsAg.Methods：A model of in vitro anti-HBV was set by using 2.2.15 cell strain,and sure the concentration of extraction to cell that had nonpoisonous side effect into CMV experiment.Then,put the concentration of extraction into cell culture,after the fourth day and the eighty day fetched the supernatant of the cell culture,used the way of solid-phase radioimmunoassay assay the content of HBsAg and HBeAg.Results：The concentration of Zuoguiwan extraction 25,12.5,6.25,3.125mg/ml had obvious inhibition effect of HBsAg and HBeAg.Each concentration of Zuoguiwan extraction had dose-effect relationship.the concentration of Zuoguiwan extraction made inhibition effect of HBeAg better than HBsAg.Conclusion：The Zuoguiwan extraction have conspicuous inhibition effect to HBV-M of 2.2.15 cell.
出处
《中西医结合肝病杂志》
CAS
2010年第2期89-91,94,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
基金
国家自然科学基金(No.90709041
30672590
30271562
30371787)
国家科技重大基础研究项目(No
2002CCC00300)
国家"十一五"重大专项"慢性重型肝炎证候规律及中西医结合治疗方案研究"(No.2008ZX10005-007)
