摘要
背景与目的:非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中转录因子Elf-1与凋亡抑制因子survivin的表达均与血管内皮生长因子(vascular endothelial growth factor,VEGF)有关,且二者均是和细胞周期有关的因子。因此本研究检测NSCLC组织中Elf-1、survivin和CD105标记的瘤内微血管密度(intra-tumor microvessel density,IMVD)的表达,探讨三者在NSCLC中表达的意义及关系。方法:采用免疫组化Power Vision TM-9000法检测60例NSCLC组织的组织芯片及9例正常肺组织中Elf-1、survivin和IMVD的表达水平;Western blot检测17例NSCLC组织及5例正常肺组织中Elf-1、survivin的蛋白表达。结果:在正常肺组织中各见1例有Elf-1和survivin弱阳性表达,余为阴性,在NSCLC组织中表达的阳性率分别为70.0%和65.0%,其表达水平与肿瘤细胞分化程度、淋巴结转移、临床分期和术后生存期有关(全部P<0.05)。Kaplan-Meier生存分析表明二者的过表达均与患者的生存率有关,阳性表达的患者生存率明显低于阴性者(P<0.01)。Kendell相关分析显示,在NSCLC组织中,二者的表达呈正相关(r=0.769,P<0.01);Spearman相关分析表明,CD105标记的IMVD与Elf-1、survivin的表达均呈正相关(r=0.446,P<0.01;r=0.435,P<0.01)。结论:Elf-1、survivin在NSCLC组织中的过表达与肿瘤细胞的分化程度、转移和预后均有关,联合检测其表达水平可作为判定NSCLC恶性生物学行为的参考指标;二者的表达均与IMVD有关,阻断其作用或可成为抗肿瘤血管生成的新途径。
Background and Objective：The expression of transcription factor Elf-1 and inhibitor of apoptosis survivin in non-small cell lung cancer （NSCLC） is correlated with the angiogenic factor vascular endothelial growth factor （VEGF）,and are both factors affecting the cell cycle. This study investigated the expression of Elf-1,survivin,and intratumoral microvessel density （iMVD） assessed by monoclonal antibody CD105 in NSCLC,and explored their correlations with clinicopathologic features and angiogenesis of NSCLC. Methods：PowerVisionTM-9000 immunohistochemistry was used to evaluate the expression of Elf-1,survivin,and CD105 in tissue microarrays containing 60 specimens of NSCLC and 9 specimens of normal tissue. Western blot analysis was used to evaluate the protein levels of Elf-1 and survivin in 17 specimens of NSCLC and 5 specimens of normal tissue. Results：Elf-1 and survivin were detected in 1 of the 9 normal tissues. The positive rates of Elf-1 and survivin in NSCLC were 70.0% and 65.0%,respectively. The expression levels of both Elf-1 and survivin were significantly related to tumor differentiation,lymphatic metastasis,clinical stage,and postoperative survival time （ P〈0.05）. Overexpression of both were related to poor prognosis：the survival rates were significantly lower in patients with positive expression than in those with negative expression （ P〈0.01）. Elf-1 expression was positively correlated with survivin expression （ r=0.769,P〈0.01）. Elf-1 and survivin expressions were positively correlated with iMVD （ r=0.446,P〈0.01; r=0.435,P〈0.01）. Conclusions：The expression of Elf-1 and survivin in NSCLC is related to differentiation,lymphatic metastasis,clinical stage,and prognosis,and both are positively correlated with iMVD. Detection their combined expression can help to predict the malignant behavior of NSCLC. Blocking the activity of Elf-1 and survivin may be a new way to inhibit angiogenesis in NSCLC.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2010年第4期434-441,共8页
Chinese Journal of Cancer
