摘要
背景与目的:侵袭转移是导致结肠癌死亡率增加的主要原因,如何阻断肿瘤的侵袭转移成为目前研究的热点。本研究旨在检测Syndecan-1基因和HPA-1基因在结肠癌组织中的表达水平,探讨二者与结肠癌侵袭转移及预后的关系。方法:用实时荧光定量逆转录聚合酶链反应(RT-PCR)分别检测49例结肠癌、49例配对的癌旁组织(距癌2cm)和49例配对远离结肠癌的手术切缘正常组织(距癌5cm以上)的Syndecan-1和HPA-1基因的表达水平,分析二者与结肠癌临床病理特征的关系。结果:结肠癌中HPA-1mRNA的表达水平(40.56±11.75)显著高于癌旁组织(18.28±11.33)和正常组织(10.80±10.20)(P均<0.001),癌旁组织明显高于正常组织(P<0.05);正常结肠组织中Syndecan-1mRNA表达水平(61.21±12.96)显著高于癌旁组织(14.35±11.06)和结肠癌组织(10.12±8.58)(P均<0.001),癌旁组织明显高于结肠癌组织(P<0.05)。Syndecan-1mRNA的表达减弱和HPA-1的表达增强与肿瘤的分化程度、浸润深度、淋巴结转移、远处转移及TNM分期显著相关(P均<0.05)。Spearman等级相关分析证明了Syndecan-1mRNA和HPA-1的表达呈明显负相关(r=-0.405,P<0.05)。结论:Syndecan-1mRNA在结肠癌组织中呈低表达,HPA-1mRNA在结肠癌组织中呈高表达,且Syndecan-1mRNA的表达减弱与HPA-1的表达增强可促进结肠癌的侵袭转移。联合检测Syndecan-1mRNA及HPA-1mRNA对于指导结肠癌临床治疗及判断预后有重要价值。
Background and Objective： Invasion and metastasis are the most common causes of mortality for patients with colorectal neoplasms,and blocking invasion and metastasis in a timely fashion has become a hot research focus. We investigated the expression of the messenger RNA（mRNA） of Syndecan-1 and HPA-1 in colorectal cancer,and their correlation with invasion and metastasis. Methods： Real-time fluorescent quantitative reverse transcription polymerase chain reaction （RT-PCR） was used to detect the expression of Syndecan-1 and HPA-1 in specimens from 49 patients with colorectal cancer,49 paired adjacent colorectal neoplasms （2 cm from the carcinoma）,and 49 surgical margins of paired normal colorectal mucosa tissue （5 cm from the carcinoma）,to analyze their correlation with clinicopathologic characteristics of colorectal neoplasm. Results： The expression of HPA-1 mRNA was significantly higher in colorectal cancer （40.56±11.75） than that in the paired adjacent colorectal neoplasms （18.28±11.33） and normal colorectal mucosa tissue （10.80±1020） （all P〈0.001）. The expression of HPA-1 mRNA was significantly higher in paired adjacent colorectal neoplasms than that in normal colorectal mucosa （ P〈0.05）. The expression of Syndecan-1 mRNA was significantly higher in normal colorectal mucosa （61.21±12.96） than in the paired adjacent mucosa （14.35±11.06） or colorectal cancer （10.12±8.58） （all P〈0.001）. The expression of Syndecan-1 mRNA was significantly higher in the paired adjacent mucosa than that in colorectal cancer （ P〈0.05）. The decreased expression of Syndecan-1 mRNA and the increased expression of HPA-1 were closely associated with the degree of differentiation,the depth of infiltration,lymph node metastasis,vessel metastasis,and TNM staging of colorectal cancer （all P〈0.05）. Spearman rank correlation analysis demonstrated a significant correlation between Syndecan-1 and HPA-1（ r=-0.405,P〈0.05）. Conclusions： The expression of Syndecan
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2010年第3期310-315,共6页
Chinese Journal of Cancer
