摘要
目的近期研究显示,Syndecan-1和乙酰肝素酶(heparanase,HPA-1)可能参与多种恶性肿瘤的侵袭和转移,但关于两者与皮肤鳞状细胞癌(cutaneous squamous cell carcinoma,CSCC)的关系较少报道。本研究旨在探讨Syndecan-1和HPA-1在CSCC组织中的表达及其与临床病理特征的关系。方法应用免疫组化方法、蛋白质印迹法和实时荧光定量PCR技术检测2009-09-01-2014-03-31潍坊市人民医院皮肤科93例CSCC及30名正常表皮中Syndecan-1和HPA-1蛋白及mRNA的表达水平。结果正常表皮、原位鳞癌、高分化鳞癌、中分化鳞癌和低分化鳞癌中Syndecan-1强阳性率分别为70.00%(21/30)、52.28%(11/21)、37.04%(10/27)、23.33%(7/30)和6.67%(1/15),CSCC Syndecan-1表达强度显著低于正常表皮对照组,χ2=13.17,P<0.01;在不同肿瘤厚度和分化程度的鳞癌中,SDC1表达强度随肿瘤厚度的增加(χ2=11.66,P<0.01)和分化程度的降低(χ2=12.51,P<0.01)而有下降趋势;CSCC患者中伴淋巴结转移组Syndecan-1表达强度显著低于无淋巴结转移组,χ2=5.78,P<0.05。CSCC组织Syndecan-1mRNA和蛋白表达水平显著低于正常对照组,且随着组织学分级的降低而依次降低(rs值分别为0.829和0.644,均P<0.001)。正常表皮、原位鳞癌、高分化鳞癌、中分化鳞癌和低分化鳞癌中HPA-1阳性率分别为3.33%(1/30)、38.10%(8/21)、62.96%(15/27)、66.67%(20/30)和86.67%(13/15),CSCC中HPA-1表达强度显著高于对照组,χ2=29.52,P<0.01。在不同肿瘤厚度和分化程度的鳞癌中,HPA-1表达强度随肿瘤厚度的增加(χ2=4.70,P<0.05)和分化程度的降低(χ2=9.15,P<0.05)而有增加的趋势,CSCC患者中伴淋巴转移组HPA-1表达强度(80.95%)显著高于无淋巴结转移组(54.17%),χ2=4.87,P<0.05。CSCC组织HPA-1mRNA和蛋白表达水平显著高于正常对照组,且随着组织学分级的降低而依次升高(rs值分别为-0.793和-0.718,均P<0.001)。CSCC中Syndecan-1与HPA-1表达呈负相关,rs=-0.473,P<0.01。结论 Syndecan-1表达降低与
OBJECTIVE Recent studies have revealed that Syndecan 1 and Heparanase（HPA 1） may be involved in invasion and metastasis of many malignant tumors, but there are few reports about the relationship between the two mole cules expressions in cutaneous squamous cell carcinoma（CSCC）. This study aimed to investigate the expression of Syndecan 1 and heparanase in CSCC and their relationship with the clinical and pathologic characteristics of CSCC. METHODS The protein and mRNA levels of Syndecan-land HPA 1 were determined in 93 cases of CSCC specimens and 30 normal human controls by immunohistochemistry, Western blotting and quantitative real-time fluorescence-based PCR respectively. RESULTS The strong positivity rates of Syndeean-1 in normal epidermis, squamous cell careimoma in situ, well, moderately and poorly differentiated squamous cell carcinoma were 70.00 % （21/30）, 52.28 % （ 11/21 ）, 37.04 % （ 10/27 ）, 23.33%（7/30） and 6. 67% （1/15） respectively, and the strong positivity rate was significantly lower in CSCC than that in normal controls（x2=13.17,P〈0.01）. The expression of Syndecan-1 was statistically correlated with thickness and histological differentiation of CSCC（x2 was 11. 66 and 12. 51 respectively, P〈0. 01）, and there was a significant difference in the expression of Syndecan-1 between CSCC with and without lymgh node metastases（x2= 5.78 ,P〈0.05）. The positivity rates of HPA-1 in normal epidermis, squamous cell carcimoma in situ, well, moderately and poorly differentiated squamous cell carcinoma were 3.33% （1/30）, 38. 10% （8/21）, 62. 96%（15/27）, 66. 67%（20/30）, 86. 67% （13/15） respectively, and the positivity rate was significantly higher in CSCC than in normal controls （x2 = 29.52, P〈 0.01）. The expression of HPA-1 was statistically correlated with thickness and histological differentiation of CSCC （x2 was 4.70 and 9.15 respectively,P〈0.05）and significant differences were obseved in the expression rate of HPA-1 between CSCC with a
出处
《中华肿瘤防治杂志》
CAS
北大核心
2016年第12期786-793,共8页
Chinese Journal of Cancer Prevention and Treatment
基金
潍坊市科技发展计划(201302005)
