摘要
目的:分离并鉴定川芎有效部位的化学成分,建立川芎药材中藁本内酯的含量测定方法。方法:采用乙醇渗漉提取、硅胶柱色谱分离,运用1H-NMR、13C-NMR鉴定化合物结构;采用高效液相色谱(HPLC)法测定藁本内酯的含量。结果:共分离鉴定了3个化合物,分别为Z-藁本内酯、Z-6,8′,7,3′-二聚藁本内酯、Z,Z′-6,6′,7,3′a-二聚藁本内酯;药材中藁本内酯含量不低于0.70%。结论:本试验建立的分离方法可用于制备藁本内酯标准品,含量测定方法可用于川芎药材的质量控制。
OBJECTIVE：To study the chemical constitutes of Ligusticum chuanxiong and to establish the method for the content determination of ligustilide. METHODS：The compounds were extracted and percolated by ethanol. Then the samples were separated using silica gel and identified by 1H-NMR,13C-NMR data.HPLC was used to assay the contents of ligustilide. RESULTS：Three compounds were isolated and their structures were identified as Z-ligustilide,Z-6,8＇,7,3＇-diligustilide and Z,Z＇-6,6＇,7,3＇a-diligustilide. The contents of ligustilide were no less than 0.70%.CONCLUSION：The separation method can be used to prepare high-purity ligustilide reference substance. And the determination method can be used for quality control of L. chuanxiong.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第15期1381-1383,共3页
China Pharmacy
基金
国家科技支撑计划子课题(2006BAI06A14-10)
关键词
川芎
藁本内酯
提取
分离
质量控制
Ligusticum chuanxiong
Ligustilide
Extraction
Isolation
Quality control
