摘要
以苯酚作为碳源从化工厂污水样品中富集筛选出降解能力较强的降解菌CP5,该菌能利用多种芳香烃化合物生长。16SrDNA序列系统发育分析表明,该菌属于假单胞菌属(Pseudomonas)。CP5可以在48h内将500mg/L的苯酚完全降解。从该菌株中扩增获得邻苯二酚2,3-双加氧酶基因,该基因编码氨基酸序列与P.putida NCIB9816-4以及P.fluorescens PC20的双加氧酶具有最高同源度,均为99%。CP5菌株对芳香烃化合物的生物修复有较大的应用前景。
A bacterial strain CP5 was isolated from a sewage water sample of a chemical factory using enrichment cultivation with phenol as a carbon source.Several aromatic compounds could be used as carbon sources for its growth.Analysis of the 16S rDNA sequence showed that it belonged to Pseudomonas.A DNA fragment of catechol-2,3-dioxygenase gene was obtained by PCR.Its coding protein showed a sequence similarity （99%） with the dioxygenases of P.putida NCIB 9816-4 and P.fluorescens PC20.Strain CP5 has potential use in the bioremediation of environmental contamination with aromatic compounds.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2010年第2期185-188,共4页
Journal of Huazhong Agricultural University
基金
国家自然科学基金项目(30800021)
中南民族大学自然科学基金重点项目(yzz06013)资助
