摘要
以芘为唯一碳源长期驯化筛选出两株高效降解芘菌Py1和Py4,经初步鉴定均为芽孢杆菌.本文研究了Py1、Py4以及它们的等比例混合菌对芘的降解性能.结果表明:Py1对芘的降解率10 h时为88%;Py4对芘的降解率14 h时为84%;混合菌对芘的降解率8 h时为88%,并用混合菌确定了最佳降解条件为37℃和pH 7.0.研究了添加不同营养物质对降解性能的影响,水杨酸钠、醋酸钠和酵母膏对混合菌降解芘有明显的促进作用,葡萄糖的作用不明显.
Two bacterial strains Pyl and Py4 have been tamed and isolated through long cultivation with Polycyclic Aromatic Hydrocarbon-pyrene as the single carbon source, it has been proven that they are both high-efficient pyrene degrading bacteria and both Bacillus sp.. The pyrene degradation ability of separated Pyl, Py4 and the consortium of equal Py1 and Py4 was studied in this project. It was showed that pyrene degradation rate was 88 % in 10 h by Pyl; 84 % in 14 h by Py4; and 88 % in 8 h by the consortium. It was also determined that the best degradation temperature and pH were 7.0 and 37 ℃ respectively. The influence of different nutrient substrates added in the degradation experiments was also studied. It was showed that sodium salicylate, sodium acetate and yeast extract had obvious stimulative effect, but glucose had no obvious effect.
出处
《南开大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第2期71-74,共4页
Acta Scientiarum Naturalium Universitatis Nankaiensis
基金
国家自然科学基金(402173036)
关键词
多环芳烃
芘
芽孢杆菌
polycyclic aromatic hydrocarbons
pyrene
bacillus sp.
