摘要
以丝瓜藤为添加物,添加到掺有黄粉虫幼虫的察氏培养基中,采用平板培养法测定白僵菌菌株的生长速度、菌落形态特征、孢子大小、产孢量、孢子萌发率及POD酶活性。结果表明:丝瓜藤能有效地抑制白僵菌的生长速率,并且与丝瓜藤的添加量成正相关;且对白僵菌的孢子大小等方面有显著促进作用。400倍处理对孢子大小的效果最佳,高达2.75000±0.1936μm;25倍处理对产孢量及孢子萌发率效果最好,分别高达(5.59±0.2755)×108个/cm2(、0.9668±4.0475)%;12.5倍处理后的白僵菌POD酶活性最高,高达0.2053±0.0013;与对照相比,均达到了极显著水平。
Luffa vine was used as additives and added into Czapek＇s medium with tenebrio molitor larvae. The colony growth speed, colony shape feature, spore size, sporulation quantity, spore germination and POD activity of Beauveria bassiana was observed with the plate culture method. The results showed that it couLd effectively inhibit the growth rate of Beauveria bassiana, and the growth rate was positive correlation with luffa addition;but it exhibited make promotion against spore size ect. The 400x was the best effect on spores size, reached （2. 75000±0. 1936） μm;the best effect on sporulation quantity and spore germination were the 20x, which were reached （5.59±0. 2755）× 10^8 ind/cm^2 ,（0. 9668± 4. 0475） % ; the 12.5x was the highest POD activity of Beauveria bassiana, reached 0. 2053 ±0. 0013 ; Compared to the control, they all reached extremely significant level.
出处
《北方园艺》
CAS
北大核心
2010年第5期161-163,共3页
Northern Horticulture
基金
河北省科技厅资助项目(06221004)
河北省教委科研资助项目(2003308)
秦皇岛市科委资助项目
关键词
丝瓜藤
白僵菌
指标测定
Beauveria bassiana
Luffa
measured leaf meal
