摘要
:3株球孢白僵菌 (Beauveriabassiana)Bb 0 2 ,Bb 0 3和Bb 0 7在不同温度 ,pH值和培养基上培养 ,结果表明 ,三者的最适生长温度在 2 5℃～ 30℃间 ;Bb 0 2和Bb 0 7在pH4.0～ 6 .0生长良好 ;适量的蛋白胨或酵母浸膏可促进菌株的生长 ,但对葡萄糖和麦芽糖的利用能力没有明显差异 ,三者在PPDA ,SDAY和SMAY上生长良好。生物测定表明 ,3菌株对小麦蚜虫均具有较强的毒力 ,但各菌株的最适剂量略有差异 ,其中Bb 0 2以 10 6mL-1较佳 ,Bb 0 3以 10 7mL-1最好 ,Bb0 7则介于 10 7mL-1～ 10
Three isolates of Beauveria bassiana, Bb 02,Bb 03 and Bb 07,were cultured at different temperature and pH and on different media.The optimum temperature for their growth ranged from 25℃ to 30℃ and Bb 02 and Bb 07 grew well at pH 4.0～6.0.The addition of peptone and yeast extract stimulated their growth,while glucose and maltose had little promoting effect.All the three isolates grew well on the media PPDA,SDAY and SMAY.Bioassay showed that they were strongly virulent against whest aphids,though their optimum dosage varied a little.The opimum concentration was 10 6/mL for Bb 02,10 7/mL for Bb 03 and 10 7～10 8/mL for Bb 07.
出处
《西南农业大学学报（自然科学版）》
CSCD
北大核心
2001年第2期144-146,共3页
Journal of Southwest Agricultural University
基金
重庆市科委攻关项目资助
