摘要
目的探讨慢性疲劳综合征的神经免疫机制及运动的干预作用。方法40只雄性Sprague-Daw ley大鼠随机分为对照组,模型组、运动+模型组及运动组;采用睡眠剥夺加负重力竭性游泳方式建立慢性疲劳综合征模型;采用8周跑轮运动作为自动体力运动模型;用高效液相色谱法检测海马、枕叶皮层5-羟色胺(5-HT)含量,应用酶联免疫吸附(ELISA)法检测血清干扰素-γ(IFN-γ)和白细胞介素-4(IL-4)水平以及IFN-γ/IL-4比值变化。结果模型组海马、枕叶皮层5-HT(11.28±5.13),(1.15±0.34)ng/g较对照组(33.56±6.42),(3.49±0.42)ng/g明显降低(P<0.01);血清IFN-γ(19.36±4.34)pg/mL及IFN-γ/IL-4比值(0.96±0.62)较对照组明显下降(P<0.01);与模型组比较,运动+模型组5-HT(23.35±4.36),(2.67±0.51)ng/g明显增加;IFN-γ水平(37.58±5.13)pg/mL和IFN-γ/IL-4比值(2.65±0.91)明显增高(P<0.05);与对照组比较,运动组5-HT水平(54.27±5.31),(5.87±0.63)ng/g升高(P<0.05)。结论长期体力运动可作为一种有效的防护措施,可降低慢性疲劳对机体神经免疫功能的损害。
Objective To probe into the neuro-immune mechanism of chronic fatigue syndrome and the intervention effects of physical exercise.Methods Forty male SD rats were randomly divided into 4 groups：control group,model group,exercise model group and exercise group.The rat model of chronic fatigue syndrome was made by sleep deprivation and overload exhaustive swimming.Exercise model was made with 8-week spontaneous wheel running.The concentration of 5-hydroxytryptamine（5-HT） in hippocampus and occipital cortex was measured with high performance liquid chromatography.ELISA was employed to measure levels of interferon-γ（IFN-γ） and interleukine-4（IL-4） in serum,and the ratio of IFN-γ/IL-4.Results Compared with the control group,the concentration of 5-HT,the level of IFN-γ and the ratio of IFN-γ/IL-4 were significantly decreased in model group（P0.01）.Compared with the model group,rats in exercise model group had higher levels of 5-HT,IFN-γ and a higher ratio of IFN-γ/IL-4（P0.05）.The concertration of 5-HT was significantly increased in the exercise group than that of in the control group（P0.05）.Conclusion As an effective protection measure,long-term exercise can lower neuroimmunity injury caused by chronic fatigue syndrome.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2010年第3期374-375,共2页
Chinese Journal of Public Health
基金
四川省科技厅青年基金(D1006)
