摘要
利用单孢稀释法,显微镜下观察分离得到72个杏鲍菇有性孢子单核体,通过单核两两交配,观察到3种典型的菌落形态,初步得到4种不同的交配型单核菌株;应用ISSR分子标记技术,对初步确定的四种不同交配型单核菌丝进行DNA指纹分析,筛选得到7条引物进行扩增,结果显示共计扩增出56条清晰易辨的多态性DNA条带,其中4个菌株共有条带9条,多态性条带47条,结果表明所得到的单核菌株确定为4种不同的交配型;ISSR分子标记技术可以作为食用菌单核菌株鉴别及指纹分析的有效工具。
Using the method of single spore dilution,72 monocytes were isolated from the microscopic observation. Through the mating between every two groups of single core strains,3 kinds of typical colony morphylogy were observed and four different mating types of monocyte strains were obtained. Analysis of DNA fingerprinting for those four types of strains was investigated by ISSR molecular marker technology,and 7 pairs of primers were screened out for amplification. The results showed that 56 DNA polymorphism bands were amplified clearly and easily,among which 9 bands were all observed in the four types of strains and 47 polymorphic bands were observed. It was indicated that the single-core strain was identified as four different strains of the mating type. ISSR molecular markers can be used as an effective tool for identification of single-core strain and fingerprint analysis of edible fungus strains.
出处
《浙江农业学报》
CSCD
北大核心
2010年第1期100-104,共5页
Acta Agriculturae Zhejiangensis
基金
浙江省重大科技专项(2007C12002)
关键词
杏鲍菇
交配型
菌落形态
ISSR
Pleurotus eryngii
mating type
colony morphology
ISSR
