摘要
为实现黑木耳菌株快速鉴定和栽培性状定向育种,研究通过荧光核染色筛选,酯酶同工酶酶谱比较及RAPD分子鉴定,筛选出了黑木耳杂交单核体的交配核型及杂交新种,并对杂交新种进行了遗传分析和栽培比较。结果显示,2个亲本筛选出4种不同类型原生质体单核体,8808亲本单核体为A1、A2型,981亲本单核体为B1、B2型。单核体单-单杂交获得核型为A1B2的杂交菌株,命名为Z1。酯酶同工酶谱及RAPD图谱结果显示Z1菌株含有来源单核的互补型条带和亲本条带。聚类分析将被试菌株分为3类,Z1菌株与981相似度高于8808菌株。栽培试验显示Z1与981栽培性状更近似而又集合了双亲的性状优势。研究的开展可为将来黑木耳菌株鉴定及杂交子遗传分析等提供快速、可靠检测方法。
For the realization of quick identification and directive b^eeding of Auricularia auricula-judae, hybrid mononuclear mating karyotype and new hybrid species were obtained with selection of fluorescence nuclear staining, comparison of esterase isozyme and molecular identification of RAPD, and genetic analysis and cultivation comparison for new hybrid species were also conducted. The results showed that four different types of protoplast monokaryons were selected from two parent monokaryons strains, which included A1, A2 type from ＇8808＇ and B1, B2 type from ＇981＇ The hybrid strain of A1B2 was named ＇ Z1 ＇ The results of esterase isozyme zymogram and RAPD molecular maps showed that the hybrid strain ＇ Z1 ＇ contained the complementary bands of monocaryon and the parent bands. The tested strains were divided into three types by cluster analysis. The similarity of strain ＇ Z1 ＇ and strain ＇ 981 ＇ is higher than that of strain ＇ Z1 ＇ and ＇ 8808 ＇. The results of cultivation experiment showed that the cultivation characters of ＇ Z1 ＇ was similar to that of ＇981 ＇ and also gathered the advantages of both parents. The research will provide a rapid and reliable detection method for the identification and hybrid-offspring genetic analysis of Auricularia auricula-judae in the future.
出处
《核农学报》
CAS
CSCD
北大核心
2015年第5期843-848,共6页
Journal of Nuclear Agricultural Sciences
基金
现代农业产业技术体系建设专项资金(CARS-24)
黑龙江省杰出青年科学基金(JC201316)
黑龙江省科学院预研项目(YYY13ZJC15)
关键词
黑木耳
原生质体单核体
RAPD
单-单杂交
栽培性状
Auricularia auricula-judae, protoplast monokaryon, RAPD polymorphism, mon-mon mating, cultivation character
