摘要
目的:优化龙脑樟愈伤组织的诱导条件。方法:采用植物组织培养方法和气相色谱方法进行。结果:MS培养基中添加不同浓度配比的激素,愈伤组织的诱导效果不同,愈伤细胞生长状况也不一样,其中在培养基上添加2,4-D4 mg.L-1+6-BA 0.2 mg.L-1愈伤组织的诱导率最高,细胞生长旺盛。同时,发现采用嫩叶为外植体诱导出的愈伤组织中含有龙脑,而采用茎为外植体诱导出的愈伤组织中不含龙脑。结论:龙脑樟愈伤组织诱导适宜的培养基为MS+2,4-D 4 mg.L-1+6-BA 0.2 mg.L-1,龙脑的生成则以嫩叶为外植体。
Objective： To optimize the condition of callus of Cinnamonum camphora induced. Method： GC and plant tissue culture method were applied in the study. Result： The effect of callus induced and the growth of callus were different in MS medium with different proportion of hormone. The ration of callus induced was the highest and the growth of callus was the most prosperous in the MS medium with 4 mg · L^-1 2,4-D and 0.2 mg· L^-1 6-BA. It is found that callus induced by young leaf contained borneol, but callus induced by young stem not. Conclusion： The optimization of callus of C. camphora induced is using the MS medium with 4 mg · L^-1 2,4-D ＋0.2 mg · L^-1 6-BA. Callus induced by young leaf can generate borneol.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第5期558-560,共3页
China Journal of Chinese Materia Medica
基金
国家自然科学基金青年科学基金项目(30901963)
中国中医科学院院自选课题(Z9)
关键词
龙脑樟
愈伤组织诱导
龙脑
Cinnamonum camphora
callus induction
borneol
