摘要
目的观察天山花楸叶总黄酮对大鼠心肌缺血/再灌注(I/R)损伤的保护作用,探讨其作用机制。方法采用改良的Langendorff逆行恒压灌流方法,建立大鼠离体心脏I/R损伤模型,观察天山花楸叶总黄酮(4.0、6.0mg·L-1)对心脏I/R损伤后心功能、心肌组织中超氧岐化酶(SOD)活性和丙二醛(MDA)含量变化的影响。利用DPPH、羟自由基、超氧阴离子、脂质过氧化反应体系,检测了天山花楸叶总黄酮(6.25、12.5、25、50、100mg·L-1)体外抗氧化能力。结果天山花楸叶总黄酮(6.0mg·L-1)可明显改善离体心脏I/R损伤后左心室发展压(LVDP)和左室压力升高或降低最大速率(±dp/dtmax),明显增加冠脉流量;天山花楸叶总黄酮(6.0mg·L-1)处理后I/R损伤心肌组织中SOD活性升高,MDA含量减少。天山花楸叶总黄酮(6.25～100mg.L-1)可浓度依赖性地清除DPPH自由基、羟自由基和超氧阴离子自由基,并抑制脂质过氧化反应。结论天山花楸叶总黄酮对心肌缺血/再灌注损伤具有明显保护作用,与其具有较强的抗氧化活性有关。
Aim To investigate the protective effect and the underline mechanisms of total flavonoids from Sorbus Tianschanica L leaf （TFST） against myocardial ischemia/reperfusion （I/R） injury. Methods The I/ R injury model of rat isolated heart was prepared by improved Langendorff retrograde perfusion method. Following the treatment, the coronary blood flow levels （CF） , left ventrieular developed pressure （LVDP） and maximal rise/fall rate of left ventrieular pressure （ ± dp/dtmax ） were monitored as the myocardial function. The superoxide dismutase （SOD） activity and malondialdehyde （MDA） content in myocardial tissue were measured by the commercial kits. The in vitro anti-oxidative capacity of TFST was detected by the DPPH and lipid peroxidation reaction system. Results Compared with I/R injury group, pre-incubation with TFST （6.0mg· L^-1） significantly improved the LVDP, ± dp/dtmax and CF during reperfusion. TFST （6. 0 mg · L^-l） treatment significantly increased the SOD activities and reduced the MDA levels in myocardium tissue. Moreover,TFST （from 6. 25 to 100. 0 mg · L^-1 ） scavenged the DPPH, hydroxyl radical and superoxide anion free radicals, and inhibited the lipid peroxidation reaction in a concentration-dependent way. Conclusions All the results demonstrate that TFST possesses the cardioprotective effect on ischemia/reperfusion injury. This efficacy may be due to its antioxidative activity.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2010年第2期251-254,共4页
Chinese Pharmacological Bulletin
基金
新疆建设兵团科技攻关计划资助项目(No2006GG52&)
新疆生产建设兵团博士资金资助项目(No2008GC-12)
关键词
天山花楸
总黄酮
大鼠
心肌
缺血/再灌注损伤
抗氧化
Sorbus Tianschanica L
total flavonoids
rats
myocardial
ischemia/reperfusion injury
antioxidative
