摘要
目的建立HPLC梯度洗脱法测定天山花楸叶与果中绿原酸含量的方法。方法采用ZORBAS ODS C18（4．6mm×15cm，5μm）色谱柱，甲醇-0．02％磷酸溶液为流动相（梯度洗脱），流速为1．0ml·min^-1，检测波长为326nm。结果绿原酸线性范围为3．952～19．76μg/ml，叶中绿原酸含量1．982％（RSD=0．58％，n=3），平均回收率为99．42％；果中绿原酸含量2．259％（RSD=1．2％，n=3），平均回收率为99．32。结论该方法经济、简便、快速、准确、可靠，可用于该药材的质量控制。
Objective To establish methods for determination of chlorogenic acid in Sorbus tianschanica. Methods The HPLC system consisted of the column of ODS ZORBAS ODS （4.6 mm × 15 cm,5 μm） column and MeOH -0.02% H3 PO3 as the mobile phase. The flow rate was 1.0 ml · min^-1. The detection wavelength was 326 nm. Results The method was linear within the range of 3. 952 - 19.76 μg/ml. Chlorogenie acid in the leaf of S. tianschanica was 1. 982% （RSD% = 0.58, n = 3 ） and the average recovery was 99.42%. Chlorogenie acid in the fruit of S. tianschanica was 2. 259% （ RSD% = 1.2, n = 3 ） and the average recovery was 99.32%. Conclusion The method is simple, sensitive, quick and highly reproductive and it is suitable for analysis of chlorogenie acid in S. tianschanica.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2008年第1期59-60,共2页
Lishizhen Medicine and Materia Medica Research
基金
新疆建设兵团科技攻关计划医药卫生专项资金资助项目(No.2006GG52)
