摘要
目的:诱变筛选阿魏菇多糖高产菌,探索食用菌的离子束和激光复合诱变育种方法。方法:尝试用阿魏菇菌丝单细胞为靶材,以离子束注入和激光辐照为复合诱变手段,采用营养缺陷型筛选办法定性初筛,摇瓶发酵定量复筛。结果:通过离子束注入诱变,获得了2株菌丝体多糖产量分别达到551.80mg/L和659.46mg/L、较1号出发菌株提高了46.5%和75.2%的多糖高产菌PFPH-1和PFPH-2;在此基础上,以激光辐照为复合诱变手段,获得了1株菌丝体多糖产量达到762.50 mg/L、较出发菌株PFPH-2提高了15.63%的多糖高产菌PFPH-3。结论:离子束和激光复合诱变育种方法在改良阿魏菇多糖高产性状方面诱变功效显著。
Objective：To obtain Pleurotus polysaccharide high -yield strains and explore an ion beam and laser composite mutagenesis breeding method for edible fungus. Method： Mycelium mono - cells of Asafoetida mushroom were treated tentatively by ion beam and laser composite mutagenesis through fermentation quantitative screening after auxotrophy qualitative primary screening. Result：Two polysaccha- ride high - yield strains, PFPH - 1 and PFPH - 2, were selected by ion beam implantation. The polysaccharide yield of these mutants was 551.80mg/L and 659.46mg/L, which were increased by 46.5% and 75.2% compared to that of initial strain No. 1. On this basis, a polysaccharide high - yield strain named PFPH - 3 was obtained by laser eradiation as composite mutagenesis method. The polysaccharide was 762.50mg/L, which was increased by 15.63% compared to that of initial strain PFPH -2. Conclusion： In this study, the mutagenic effect was significant that ion beam and laser composite mutagenesis breeding method was used in improving Pleurotus polysaccharide yield.
出处
《生物技术》
CAS
CSCD
北大核心
2010年第1期30-33,共4页
Biotechnology
基金
新疆大学青年教师科研启动基金项目("阿魏菇多糖高产菌株的复合诱变育种及发酵过程优化研究
"070295)
国家发改委高技术产业化项目("新疆特色食用菌离子束注入诱变技术及其深加工高技术产业化
"[2004]2077)资助
关键词
阿魏菇多糖
高产菌
离子束
激光
复合诱变育种
Pleurotus polysaccharide
high - yield strains
ion beam
laser
composite mutagenesis breeding
