摘要
目的:建立以高效液相色谱法测定痛经康胶囊中延胡索乙素的含量方法。方法:色谱柱为AgilentHypersil ODS C18(250mm×4.6mm,5μm),流动相为甲醇-0.04%磷酸溶液(50∶50),流速为1.0mL·min-1,检测波长为285nm,柱温30℃。结果:延胡索乙素检测浓度在0.0245-0.245mg·mL-1范围内的线性关系良好(r=0.9999);平均回收率为98.26%,RSD=1.10%(n=6)。结论:该方法简便、快速、准确,可用于痛经康胶囊的质量控制。
Objective：To establish a HPLC method for the determination of tetrahydropalmatine in Tongjingkang Capsule.Methods：the determination was performed on Agilent Hypersil ODS C18 with the mobile phase consisted of methanol-0.04% H3PO4（50∶ 50） at a flow rate of 1.0 mL·min^-1.The detection wavelength was 285 nm and the colum temperature was 30℃.Results：The linear relation of tetrahydropalmatine was excellent within the range of 0.0245-0.245mg·mL^-1（r=0.9999）,and the average Recovery was 98.26% ,RSD=1.10%（n=6）.Conclusion：The method is simple,rapid,accurate,and Can be used for the quality control of Tongjingkangcapsule.
出处
《中医学报》
CAS
2010年第1期108-109,共2页
Acta Chinese Medicine
