摘要
在全面调查昆仑山北坡前山带塔里木沙拐枣(Calligonumroborovskii A.Los.)分布的基础上,设置3个海拔梯度:A1(2190m)、A2(2355m)、A3(2495m),对不同海拔梯度塔里木沙拐枣的生理生态特性进行研究。结果显示:叶绿素a(Chla)、叶绿素b(Chlb)和总叶绿素(chl(a+b))含量均随着海拔的上升而增大,高海拔A3与低海拔A1相比,Chla、Chlb和Chl(a+b)含量分别增大了48.30%、40.10%和43.71%,差异均达显著水平(P<0.05)。SLA和Nmass随着海拔的升高都增大,A3与A1相比分别增大了33.99%和20.97%,差异达到显著水平(P<0.05)。LMA随着海拔升高而减小,A3与A1相比减小了30.15%,差异达到显著水平(P<0.05)。丙二醛(MDA)含量和质膜透性(MP)变化较为一致,随着海拔上升而减小,A3与A1相比分别减小了184.06%和58.33%,差异均达显著水平(P<0.05),说明在A1受到的伤害更大。类胡萝卜素(Car)、脯氨酸(Pro)和抗坏血酸(AsA)含量随着海拔的上升呈下降趋势,A3与A1相比分别下降了65.88%、290.21%和38.97%,差异均达显著水平(P<0.05),说明A1处非酶类保护物质含量最高。酶保护系统中的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX),只有CAT活性随海拔上升而升高,其余3种酶的活性均随着海拔的升高而降低,A3与A1相比分别降低了18.75%、122.37%、23.03%%,差异均达到显著水平(P<0.05)。与此同时,随着海拔的升高,超氧阴离子自由基(O2-)和过氧化氢(H2O2)含量也呈下降趋势,A3与A1相比分别下降了54.48%、9.69%,差异达显著水平(P<0.05)。在整个研究区域,AOS维持在低浓度范围,而低浓度AOS正好诱导防御基因表达,及时清除活性氧,另外非酶类保护物质含量的增加也有利于清除细胞内的活性氧,维持细胞膜的稳定性,从而保证塔里木沙拐枣正常的生理功能。
We investigated the ecophysiological responses of Calligonum roborovskii at different altitudes in the north of Kunlun Mountain,to determine ecophysiological adaptation mechanisms of the species antioxidative system.So we studied the content of Chla,Chlb,Chl（a＋b） and Carotenoid,malondialdehyde（MDA）,ascorbate（AsA） and proline（Pro）,the membrane permeability（MP）,specific leaf area（SLA）,LMA,Nmass,and the activities of peroxidase（POD）,catalase（CAT）,superoxide dismutase（SOD） and ascorbate peroxidase（APX） in leaves of C.roborovskii from different altitudes（A1：2190m,A2：2355m,A3：2495m） in Kunlun Mountain.The results showed that Chla,Chlb and Chl（a＋b） increased with increasing altitude.At A3,they increased 48.30%,40.10% and 43.71%,respectively,compared with that in A1.SLA and Nmass also increased with increasing altitude.At A3,they increased 33.99% and 20.97%.But LMA decreased with increasing altitude.At A3,they decreased 30.15%.MP and content of MDA had a similar change：they decreased with increasing altitude.Compared with A1,they decreased 184.06% and 58.33%,indicating that it was least damaged in A1.Carotenoid,Pro and AsA decreased with increasing altitude.They decreased 65.88%,290.21%,38.97%,compared with A1.This showed non-enzyme antioxidant was more accumulated in A1.The activitity of SOD,POD and APX all decreased with increasing altitude,except CAT.Compared with A1,they decreased 18.75%,122.37%,23.03%.At the same time,content of O-2 and H2O2 also decreased with increasing altitude.They decreased 54.48% and 9.69%.In the area,Aos keep at low level,so it induced to gene expression.More non-enzyme antioxidant and stonger activity of enzyme were favourable for eliminating intracellular active oxygen,keeping the cell membrane in a stable condition and ensuring normal growth of C.roborovskii.
出处
《生态学报》
CAS
CSCD
北大核心
2010年第3期602-609,共8页
Acta Ecologica Sinica
基金
中国科学院知识创新工程重要方向资助项目(KZCX2-YW-431)
新疆科技攻差项目(200933125)
国家重点基础研究发展计划资助项目(2009CB421303)
新疆科技重大专项和科技攻关资助项目(200733144-2)
关键词
塔里木沙拐枣
海拔
抗氧化系统
生理生态
昆仑山
Calligonum roborovskii altitude antioxidative system physioecological Kunlun Mountain
