摘要
以云南玉龙雪山自然保护区内不同海拔梯度(A1:2 750 m,A2:2 900 m,A3:3 050 m,A4:3 200 m,A5:3 350 m,A6:3 500 m)的川滇高山栎为研究对象,通过分析其主要生理生化指标变化,探讨了该树种适应垂直气候环境变化的生理机制。结果表明,随着海拔的升高,川滇高山栎叶片各生理指标呈非线性变化。在较低海拔点(2 750 m)及较高海拔点(3 500 m)的叶绿素含量均显著降低,而在中间海拔点(3 200 m)的叶绿素含量增加;在海拔3 050 m处,膜脂过氧化产物MDA含量、脯胺酸含量、可溶性糖含量、抗氧化酶(SOD、CAT、APX)活性等指标均处于最低水平,而当海拔超过或低于3 050 m时,其值都有所增加。海拔3 050 m是川滇高山栎的最适生长区域,光照充足,叶绿素含量较多,生长较快,膜脂过氧化程度轻;在低海拔区域,由于光照不足或人为破坏,导致其生长较缓慢;在高海拔区域,虽然该物种能通过抗氧化酶活性的增加和可溶性细胞代谢物质的调节形成一定的生态适应,但随海拔的进一步增加(3 500 m),由于低温、大气稀薄或强的紫外辐射等不利环境的加剧,植株体内的抵御能力有限,亦限制了川滇高山栎的生长发育。该研究结果可为西南地区川滇高山栎天然林的保护提供一定的理论支持。
Taking Quercus aquifolioides, which grown at different altitude gradient （ A1 ： 2 750 m, A2 ： 2 900 m, A3 ： 3 050 m, A4 ： 3 200 m, A5 ： 3 350 m, A6 ： 3 500 m） of Nature Reserve in Yulong Snow Mountain as the main materials, the main physiological and biochemical indexes were studied and the physiological mechanisms of Quercus aquifolioides responses to changes of vertical climate were analyzed. The results showed that with the in- crease of altitude, there was nonlinear variation in the physiological indicators in leaves of Quercus aquifolioides. The chlorophyll contents were significantly lower at low （2 750 m） and high altitude （3 500 m）, while increased at the middle altitude （3 200 m） . In addition, it was not only the content of membrane lipid peroxidation products （MDA） , proline content and soluble sugar content, but also the activities of antioxidant enzyme （SOD, CAT and APX） reached to the lowest level at the 3 050 m altitude, while the values of these indicators were increased at oth- ers altitude. In conclusion, the 3 050 m altitude was an optimum zone for the growth of Quercus aquifolioides as a re-suit of sufficient sunlight, more content of chlorophyll, faster growth and lower level of membrane lipid peroxidation ; At the low altitude, the lack of sunlight or man-made destruction resulted the slower growth of Quercus aquifolioides ; At the high altitude, although the Quercus aquifolioides could form certain ecological adaptation by increasing the ac- tivities of antioxidant enzymes and regulating the soluble cell metabolism substance, the growth and development of Quercus aquifolioides were limited with the further increase of altitude （ reached to 3 500 m） for the seriously adverse environment, such as the low temperature, thin atmosphere and strong ultraviolet radiation. The research could pro- vide certain theoretical support to protect the natural Quercus aquifolioides forest of Southwest China.
出处
《西部林业科学》
CAS
2015年第4期1-6,24,共7页
Journal of West China Forestry Science
基金
国家自然科学青年基金项目(31100292)
云南省省级重点学科园林植物与观赏园艺建设经费项目(50097401)
西南林业大学博士启动项目(110909)
关键词
川滇高山栎
抗氧化系统
海拔
生理生化
Quercus aquifolioides
antioxidative system
altitude
physiological and biochemical
