摘要
【目的】筛选抗丝黑穗病菌3号生理小种基因的分子标记,从而实现实验室内对抗丝黑穗病的选择,免去在育种中对抗丝黑穗病的田间鉴定。【方法】本研究采用SSR技术,应用分离群体分组分析法,分别利用恢复系分离群体(2381R/矮四)和保持系分离群体(Tx622B/7050B),筛选抗丝黑穗病菌3号生理小种基因的分子标记。【结果】试验得出结果如下,高粱对丝黑穗病菌3号生理小种的抗性属于质量性状遗传,抗性表现为显性,只要亲本之一抗病,F1代即表现抗病;发现了2个在抗病品系中稳定出现、可作为高粱抗丝黑穗病菌3号生理小种基因标记应用的SSR标记:Xtxp13和Xtxp145。Xtxp13位于B染色体上,Xtxp145位于I染色体上,与抗病基因的重组率分别为9.6%和10.4%,距离抗病基因的遗传图距分别约为9.6cM和10.4cM。【结论】高粱对丝黑穗病菌3号生理小种的抗性可能受2对彼此独立的非等位基因控制,并且基因之间存在着互作;筛选SSR标记时发现,高粱抗丝黑穗病基因的分子标记较易在保持系群体中找到,在恢复系群体中DNA片段多态性较少,表明恢复系和保持系在抗性机制上可能存在差异。
[Objective] The objectives of the research are screening molecular markers of sorghum resistance gene to head smut physiological race 3 by using SSR. Once the molecular markers are found, it would become true to select resistant lines in the indoor laboratory, and the evaluation in the field may be neglected, [Method] Bulked segregation analysis （BSA） was applied in the study. Two segregation population, restorer line population （R population） 2381R/Aisi and maintainer line population （B population） Tx622B/7050B, were used in the research. [Result] The resistance to sorghum head smut physiological race 3 is a qualitative trait inheritance. It has a dominant genetic effect. F1 would be resistant, too, provided one of the parents is resistant. It is the first time getting two stable SSR markers which can be used as molecular markers of sorghum resistance genes to head smut physiological race 3, they are Xtxpl3 and Xtxp145. Xtxp13 located in linkage group B, and Xtxp145 located in linkage group I. The recombination percentage between marker and resistant gene are 9.6% and 10.4%, respectively. The genetic distances to resistant gene are 9.6 cM and 10.4 cM, respectively. [Conclusion] The resistance to sorghum head smut physiological race 3 may be controlled by two mutual independent nonallelic genes, and there is interaction between them. It is found that molecular markers are easier to find in B population than R population during the screening of SSR markers. It may show the different resistance mechanism between restorer line and maintainer line.
出处
《中国农业科学》
CAS
CSCD
北大核心
2010年第4期713-720,共8页
Scientia Agricultura Sinica
基金
国家“863”计划课题(2004AA241230)
国家“十一五”科技支撑计划课题(2006BAD02B03)
高粱产业技术体系建设(nycytx-12)
辽宁省教育厅重点实验室资助项目(20060806)
辽宁省自然科学基金项目(20022094,20061045)
沈阳市科技局国际合作项目(1091241-6-00)
