摘要
目的:建立不同产地大黄的分子系统学基础,为大黄的基源鉴定和品质评价提供分子依据。方法:采用PCR技术获得ITS基因,进行测序,经软件进行统计分析,计算各样本间的遗传距离并建立系统树。结果:大黄ITS序列G+C含量较高,达66.55%～68.49%,8个样品ITS(包括5.8s)序列的长度范围为562～568。所有样品5.8srDNA高度一致,除DH5样品有两个位点的碱基转换外,其余无碱基差异。结论:ITS序列特征可以作为大黄种间鉴别的有效分子标记,药用大黄在属内与其它种关系较远。
Objective：To establish rhubarb from different areas the basis of molecular systematics so as to offer molecular basis for identification of Rhubarb-based source and evaluation of quality.Methods：The ITS gene of rhubarb was obtained by PCR and sequenced to be analyzed by DNAStar software to calculate the genetic distance among different specimens and to establish the system tree.Results：The ITS sequence of Rhubarb have high＂G＋C＂ bases content between 66.55%~68.49% in total DNA of Rhubarb.The length of 8 ITS sequence（including 5.8s） are in the range of 562-568 bases.The 5.8 srDNA of all Rhubarb specimens reflect a high degree of consensus,and apart from two sites of the DH5 specimen converted interactively,all other bases remains no differences.Conclusion：The ITS sequence characteristics could be recognized as effective molecular marker,and the relation between Medicinal rhubarb Species and other rhubarb Species are distant within rhubarb class.
出处
《辽宁中医杂志》
CAS
北大核心
2010年第1期136-138,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
甘肃省教育厅科研项目(014-02)
关键词
大黄
ITS
序列分析
道地性
rhubarb
internal transcribed spacer
sequence analysis
geoherbalism
