摘要
目的:建立甘肃当归功效的分子系统学基础,为甘肃当归"道地性"提供分子依据。方法:采用PCR技术获得ITS基因,进行测序,经软件进行统计分析,计算各样本间的遗传距离并采用距离矩阵法构建NJ系统发育树,分析种群间的关系。结果:甘肃岷、漳两县当归ITS序列差异极小,分子进化分析显示岷漳两县产当归与欧当归的关系较近,但不与当归属其他植物聚合,是当归属中一个特殊类群。结论I:TS序列特征可以作为当归种间鉴别的有效分子标记,岷漳两县这种特殊的环境正是"岷当归"这一特殊种群形成的主要原因。
Objective：To establish molecular systematics basis of the Angelica efficacy and provide evidence of genuine regional drug.Methods：To get ITS genes with PCR technique,and detect sequence,construct NJ system tree,and analyze and relationship.Results：the difference of ITS sequence was small between MinXian and ZhangXian county,and it showed close relationship with the europe angelica with phylogenetic analysis.Conclusion：ITS sequence could be a Angelica species identification as an effective molecular marker.The special environment of MinXian and ZhangXian County is ＂Min Angelica＂ the main reasons about the formation of the special populations.
出处
《甘肃中医》
2010年第12期68-69,共2页
Gansu Journal of Traditional Chinese Medicine
关键词
当归I
TS
序列分析
道地性
angelica
ITS
sequences analysis
genuine regional
