摘要
试验改进了菜籽粕蛋白的酶解工艺,并对改进后的工艺条件进行了优化。采用2709碱性蛋白酶水解菜籽粕蛋白,分别考察了pH值、酶用量、酶解时间、温度、底物浓度对蛋白回收率的影响规律,在此基础上,利用响应面分析法对菜籽粕蛋白的酶解条件进行了优化。结果表明,2709碱性蛋白酶水解菜籽粕蛋白的较优条件为pH11.5、温度50℃、底物浓度4%、水解时间173min、酶用量5778U·g-1,此时蛋白回收率可达87.18%。
In this paper, the technology of enzymolysis rapeseed meal protein was improved and enzymolysis conditions were optimized. Rapeseed meal protein was hydrolysised by 2709 alkaline protease. The relationship between protein recovery rate and enzymatic hydrolysis condition, such as pH, enzyme concentration, hydrolysis time, enzymolysis temperature, substrate concentration, was determined. Based on the above-mentioned measures, the condition of 2709 alkaline protease hydrolysis rapeseed meal protein was optimized by the response surface methodology. The results showed that the optimition hydrolysis condition was pHt 1.5, hydrolysis temperature 50 ℃, substrate concertation 4%, hydrolysis time 173 min, enzyme concentration 5 778 U·g^-1; in this condition, the protein recovery rate was about 87.18%.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2009年第12期101-106,共6页
Journal of Northeast Agricultural University
基金
国家博士后资助基金项目(20060390863)
哈尔滨市青年科学技术专项基金(2007RFQXN019)
关键词
菜籽粕
酶水解
碱性蛋白酶
响应面分析法
rapeseed meal
enzymatic hydrolysis
alkaline protease
response surface methodology
