摘要
[目的]为生产上提取分离火龙果果皮红色素提供技术参考。[方法]以火龙果果皮干粉为原料，用溶剂萃取法提取红色素，用大孔树脂吸附法分离红色素，进而探讨火龙果果皮红色素的提取、吸附与分离条件。[结果]20％乙醇对火龙果果皮红色素的提取效果优于20％甲醇、20％丙酮、水和0．1mol／L盐酸；当乙醇浓度≤40％时，提取效果随着乙醇浓度的提高而改善。以乙醇为浸提剂的适宜提取条件：pH值为3．0～6．0，提取温度为20～40℃，最佳料液比为1：60，提取时间为5～10min。不同型号树脂对该色素的吸附效果依次为D301〉X-5〉S-8〉D101〉D201．解吸效果依次为X-5〉D101〉D201〉D301、S-8。40％～50％乙醇宜作为解吸溶剂使用。D101和D201树脂对火龙果果皮红色素的分离富集效果优于X-5树脂。[结论]火龙果果皮红色素宜用低浓度乙醇进行萃取，宜使用D101和D201树脂进行吸附分离。
[ Objective ] The purpose was to supply technical reference for extracting and isolating red pigment from pitaya peel in production. [ Method ] With dry powder of pitaya peel as raw material, the red pigment was extracted by solvent extraction method and isolated by macro- porous resin adsorption process to discuss its extraction, adsorption and isolation conditions. [ Result ] The extraction effect on the red pigment from pitaya peel of 20% ethanol was superior to that of 20% methanol, 20% acetone, water and O. 1 mol/L hydrochloric acid. When the ethanol concn, was no higher than 40% , the extraction effect was being improved along with the increase of ethanol concn. The suitable condition of extraction with ethanol as extracting solvent was as follows ： the pH value was 3.0 - 6.0, the extraction temperature was 20 - 40 ℃, the optimum material-to-liquid ratio was 1 ： 60 and the extraction time was 5 - 10 min. The adsorption effects of resin of different types on the pigment were in sequence of D301 〉 X-5 〉 S-8 〉 D101 〉 D201 and their desorption effects were in sequence of X-5 〉 D101 〉 D201 〉 D301 ,S-8. The ethanol solution with alcohol degree of 40% -50% was suitable to be applied as desorption solvent. The isolation and enrichment effects on the red pigment from pitaya peel of the resin D101 and D201 were superior to that of the resin X-5. [ Conclusion] Red pigment from Pitaya Peel can be extracted by low-concentration ethanol, and can be absorbed and separated by D101 and D201 resin.
出处
《安徽农业科学》
CAS
北大核心
2010年第1期347-349,496,共4页
Journal of Anhui Agricultural Sciences
关键词
火龙果
色素
提取
分离
大孔树脂
Pitaya
Pigment
Extraction
Isolation
Macroporous resin
