摘要
目的:探讨临床分离铜绿假单胞菌mucA基因突变情况及其与菌落形态、藻酸盐合成之间关系。方法:对58株临床分离铜绿假单胞菌(50株黏液型菌株和8株非黏液型菌株)mucA基因进行PCR-SSCP及DNA序列分析,与GenBank中野生型铜绿假单胞菌PAO1标准序列进行比对,并用比色法测定58株细菌藻酸盐浓度。结果:细菌mucA基因突变总发生率为100%,能够影响氨基酸编码的有意义突变在非黏液型菌株中未能检获,在黏液型菌株中为32%(16/50);共发现14个突变位点,其中导致氨基酸编码发生改变的位点有5个,其余为沉默突变;黏液型菌株藻酸盐含量较非黏液型细菌显著增加(P<0.01),发生了有意义的mucA基因突变的菌株,藻酸盐合成较发生无意义突变的菌株显著增加(P<0.01)。结论:mucA突变与临床分离铜绿假单胞菌菌落形态以及藻酸盐合成能力有关。
Objective To determine the characterization of mucA gene mutation in clinically isolated Pseudomonas aeruginosa（P.aeruginosa）,and the relation between mucA mutation and the mucoid phenotype.Methods A total of 58 strains of P.aeruginosa were collected.Of them,8 were nonmucoid phenotype and 50 were mucoid phenotype.We detected mucA mutations with PCR-SSCP and sequencing analysis.Alginate was examined by colorimetry.Results All strrains had mucA mutations（100%）,16 of the 50（32%）isolates contained mucA mutations that could alter the encoding sequence of amino acids,and the rate in nonmucoid isolates was 0.Fourteen mutation sites were found,5 of which could alter the encoding sequence of amino acids,and the others were silent mutations.The alginate concentration of mucoid P.aeruginosa was higher than the nonmucoid P.aeruginos（P〈0.01）.The alginate concentration of the isolates which contained mucA mutations that could alter the encoding sequence of amino acid was higher than the strains only with silent mutations（P〈0.01）.Conclusion mucA mutation correlates with the alginate production and phenotype of bacterial colonies.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2009年第12期1196-1201,共6页
Journal of Central South University ：Medical Science
基金
湖南省科技厅计划项目(04FJ3037)~~
