摘要
目的探讨趋化因子CXCL-16对骨髓基质细胞的体外迁移作用。方法采用全骨髓法培养成年Wistar大鼠骨髓基质细胞，取第5代骨髓基质细胞行流式细胞仪鉴定；然后通过细胞免疫荧光及RT—PCR的方法检测骨髓基质细胞表达趋化因子受体CXCR6的情况，利用Transwell小室法探讨趋化因子CXCL-16对骨髓基质细胞的体外趋化作用及其特异性。结果第5代骨髓基质细胞的间充质干细胞的标志CD29的表达呈阳性，而造血干细胞表面标志CD45呈阴性；细胞免疫荧光及RT—PCR结果从基因和蛋白两方面证实骨髓基质细胞表达趋化因子受体CXCR6，趋化因子CXCL-16（5～500ng／ml）体外可趋化骨髓基质细胞迁移，抗CXCR6多克隆抗体可对抗其趋化迁移作用。结论CXCL-16／CXCR6通路参与骨髓基质细胞体外迁移，为进一步研究骨髓基质细胞的迁移机制提供了理论依据。
Objective To explore the roles of CXCL - 16 in trafficking of marrow stromal cells （MSCs） migration in vitro. Methods MSCs were isolated from whole bone marrow of adult Wistar rats and cultured to the 5th passage, then CD29 and CD45 were applied to identify them by flow cytometric. Immunofluorescence and RT -PCR were used to detect whether CXCR6 was expressed in MSCs in vitro. Chemotaxis assay was also used to detect whether the migration of MSCs was affected by CXCL - 16 in vitro. Results After the 5th passage, MSCs expressed CD29 and didn＇t express CD45. Immunofluorescence and RT - PCR showed that MSCs expressed chemokine receptors CXCR6 and CXCL - 16 （ 5,50,250, 500 ng/ml）, which could induce the migration of MSCs （ P 〈 0. 05 ） in vitro. While anti - CXCL - 16 polyclonal antibody could antagonize against its migration - inducing effect. Conclusion The migration of MSCs in vitro through the mediation of CXCL - 16/CXCR6 pathway provides theory basis for further study.
出处
《中华神经外科杂志》
CSCD
北大核心
2009年第12期1146-1149,共4页
Chinese Journal of Neurosurgery
基金
山东省自然科学基金重点项目（Z2006C02）
