摘要
目的探讨趋化因子Fractalkine对骨髓基质细胞(BMSCs)的体外迁移作用。方法采用全骨髓法培养成年Wistar大鼠BMSCs,取第五代BMSCs行免疫荧光鉴定;后通过细胞免疫荧光及RT-PCR的方法检测BMSCs表达趋化因子受体CX3CR1的情况,利用Boyden小室法探讨趋化因子Fractalkine对BMSCs的体外趋化作用及其特异性。结果第五代BMSCs都表达间充质干细胞标记物Vimentin、Laminin及Fibronectin;CX3CR1细胞免疫荧光及RT-PCR结果证实BMSCs表达趋化因子受体CX3CR1,趋化因子Fractalkine(5、50、500ng/mL)体外可趋化BMSCs迁移,抗Fractalkine多克隆抗体可对抗其趋化迁移作用。结论Fractalkine/CX3CR1通路参与BMSCs体外迁移,为进一步研究BMSCs的迁移机制提供了理论依据。
Objective To explore the roles of Fractalkine in triggering the migration of bone marrow stromal cells （BMSCs）. Methods BMSCs were isolated from Wistar rats and cultured to the 5th passage and identified by detecting Vimentin, Laminin and Fibronectin with immunofluorescence. Then immunofluorescence and RT-PCR were employed to detect the expression of CX3CR1 on BMSCs in vitro. The effect of Fractalkine on the migration of BMSCs in vitro was detected by Boyden chamber assay. Results After 5 passages, all BMSCs expressed Vimentin, Laminin and Fibronectin. Immunofluorescence and RT-PCR showed BMSCs expressed, the respective receptors for Fractalkine, CX3CR1. Fractalkine （5, 50, 500 ng/mL）, in vitro, induced the migration of BMSCs （P〈0.05）, and the polyclonal antibody against Fractalkine could suppress the migration. Conclusion In vitro, Fractalkine/CX3CR1 pathway mediates the migration of BMSCs, which offers the theoretical evidence for studying further the migration of BMSCs.
出处
《中华神经医学杂志》
CAS
CSCD
2006年第12期1205-1208,共4页
Chinese Journal of Neuromedicine
