摘要
用ＳＤ种系清醒大鼠，观察脑室注射高渗物质引起的饮水及ｃｆｏｓ在脑内的表达部位。实验结果表明，脑室内微量注射１５ｍｏｌ／Ｌ、３ｍｏｌ／ＬＮａＣｌ或３ｍｏｌ／Ｌ蔗糖均可诱导饮水反应，并在前脑的终板血管器官、正中视前核和下丘脑视上核与室旁核中见到Ｆｏｓ样免疫反应阳性细胞，同样在后脑的最后区、臂旁外侧核与孤束核中也能见到Ｆｏｓ样免疫反应阳性细胞，但在穹窿下器官却无任何诱导的Ｆｏｓ样免疫反应。渗透压相同的ＮａＣｌ（１５ｍｏｌ／Ｌ）与蔗糖（３ｍｏｌ／Ｌ）在诱导Ｆｏｓ样免疫反应阳性细胞上有量的差别（ＮａＣｌ强于蔗糖），３ｍｏｌ／ＬＮａＣｌ诱导的Ｆｏｓ反应比１５ｍｏｌ／Ｌ的强。结合以往的研究结果，本实验结果说明，中枢注射高渗物质引起的脑内Ｆｏｓ样免疫反应阳性细胞表达部位，除穹窿下器官外，与中枢注射血管紧张素Ⅱ或外周给予高渗物质的基本相同。提示：不同的致渴因子和不同的给药途径引起饮水反应的脑内通路可能是不完全相同的。
Drinking behavior and the distribution of c fos expression in the brain provoked by intracerebroventricular (icv)injection of hypertonic substances were studied in awaking Sprague Dawley rats. The results indicated that icv injection of 1 5 mol/L NaCl,3 mol/L NaCl or 3 mol/L sucrose could induce significant drinking response and Fos like immunoreactive(FLI) expression in organum vasculosum of laminae terminalis (OVLT),median preoptic nucleus (MPN) of forebrain, in supraoptic nucleus (SON) and paraventricular nucleus(PVN) of hypothalamus as well as in area postrema (AP),lateral parabrachial nucleus (LPBN) and solitary tract nucleus (STN) of hindbrain. No FLI expression was observed in subfornical organ (SFO). The induced FLI expression by hypertonic saline stimulation was stronger than that by sucrose solution with the same osmolarity.The FLI expression induced by 3 mol/L NaCl was more than that by 1 5 mol/L NaCl. As compared with previous studies, the present results indicated that except for SFO,the distribution of FLI expression in brain induced by central injection of hypertonic substances was similar to that induced by central injection of angiotensin II and peripheral injection of hypertonic substances.These results suggest that the central pathways underlying drinking response induced by different dipsogenic factors through different routes of administration are not the same.
出处
《动物学报》
SCIE
CAS
CSCD
1998年第3期308-313,共6页
ACTA ZOOLOGICA SINICA
基金
国家自然科学基金
关键词
大鼠
饮水行为
C-FOS原癌基因
脑室注射
Rats, Drinking behavior, c fos proto oncogene, Hypertonic solution, Intra cerebroventricular injection
