摘要
用聚丙烯酰胺凝胶等电聚焦电泳方法，分析了社鼠的肝、肾、心肌、骨骼肌、肺、脾和脑等多种组织器官的ＬＤＨ、ＡＤＨ、ＥＳＴ、和ＳＯＤ４种同工酶，对各组织器官的酶带数目和分布，以及酶活性进行了比较研究。结果表明，社鼠的ＬＤＨ同工酶、ＡＤＨ同工酶和ＥＳＴ同工酶具有比较明显的组织特异性，而ＳＯＤ同工酶的组织特异性较低。肺和脾除ＥＳＴ同工酶活性较高外，脑除ＬＤＨ同工酶活性较高外，其它３种同工酶的活性均较低；而肝和肾中４种同工酶的活性普遍很高。心肌和骨骼肌因氧张力不同而使ＬＤＨ同工酶酶谱存在明显差异，但其它３种同工酶酶谱却非常相似。
By the method of isoelectro focusing PAGE,four kinds of isozymes in different tissues of Rattus niviventer captured in Beijing were studied.The dispersal and the activities of the isozyme bands were compared,and distinct organ specificity were found in EST isozymes,LDH isozymes and ADH isozymes,but not in SOD isozymes. The activities of isozymes were usually much higher in liver and kidney than in the other organs,but usually lower in spleen,lung and brain,except for EST isozymes in spleen and lung and except for LDH isozymes in brain.Heart muscle and skeleton muscle are significantly differential in LDH isozymes because of their difference in oxygen tension,nevertheless,they are quite similar in other three kinds of isozymes.The differences of various organs in isozymes are in keeping with their differences in physiological functions.
出处
《兽类学报》
CAS
CSCD
北大核心
1997年第2期141-145,81,共5页
Acta Theriologica Sinica
关键词
社鼠
组织特异性
组织器官
同功酶
Rattus niviventer
LDH isozyme
ADH isozyme
EST isozyme
SOD isozyme
Tissue specificity
