摘要
以蚕豆品种临蚕2号为试验材料,采用土培法,研究了在镉胁迫期间蚕豆幼苗抗氧化酶(SOD、CAT和POD)活性、脯氨酸、丙二醛和活性氧含量的动态变化过程,以探讨外源NO和H2O2对镉毒害下蚕豆幼苗的保护效应。结果表明,0.1 mmol/L SNP(外源NO供体)和0.01 mmol/L H2O2单独处理均可缓解0.1 mmol/L镉对蚕豆幼苗的氧化损伤,而二者互作处理存在正协同效应,主要表现在增强了蚕豆幼苗的保护酶活性,其中SOD在镉胁迫72h后活性达到峰值,比对照提高57.89%,CAT和POD均在镉胁迫48 h后活性达到最大值,分别比对照提高69.60%和101.18%;镉胁迫120 h时,与单镉胁迫处理相比,互作处理的脯氨酸含量提高了134.88%,而MDA含量、内源H2O2含量和体内O2.-的产生速率下降,有效地增强了蚕豆幼苗在镉胁迫期间的抗氧化能力。
This study investigated the change of antioxidant enzymes（including SOD,CAT and POD）,proline content,MDA and reactive oxygen species（ROS） in horsebean seedlings under Cd stress,and to study the protective effect of NO and H2O2.Sodium nitro presside（SNP） was used as a NO donor.Application of SNP and H2O2,alone and in mixtures helped protect horsebean seedling leaves from Cd oxidative damage.However,treatment E showed a much better protective impact than treatments C and D,which significantly increased activities of antioxidant enzymes.Seventy two hours after Cd stress,SOD reached its maximum（57.89% over the control）.Forty eight hours after Cd stress,the activities of CAT and POD reached a maximum（69.60% and 101.18% over the control）.One hundred and twenty hours after Cd stress,the proline content in treatment E increased by 134.88% compared with treatment B,and the contents of MDA,and H2O2 were largely reduced as was the production rate of O2^·-.
出处
《草业学报》
CSCD
北大核心
2009年第6期186-191,共6页
Acta Prataculturae Sinica
关键词
一氧化氮
双氧水
蚕豆幼苗
镉胁迫
抗氧化能力
NO
H2O2
horsebean（Vicia faba） seedling
Cd stress
antioxidative capability
