摘要
目的通过建立大鼠孕期高糖模型，观察出生后早期过度喂养对子代远期代谢的影响。方法成年Wistar大鼠妊娠第5天，给予链脲霉素50mg／kg腹腔内注射，建立高糖环境模型。将子代大鼠分为3组：宫内血糖环境正常组（CP组）：仔鼠8只；宫内高血糖环境模型鼠出生仔鼠，且每只糖尿病母鼠哺乳喂养8只仔鼠（DP-1组）；宫内高血糖环境，每只糖尿病母鼠哺乳期喂养4只仔鼠（DP-2组）。观察出生后3～10周3组仔鼠体重变化，仔鼠10周龄时行口服葡萄糖耐量实验（OGTT），11周龄行高胰岛素-正葡萄糖钳夹实验，并采用酶联免疫吸附法测量胰岛素水平评价其胰岛功能。结果DP-1和DP-2组仔鼠出生后3～10周的体重增长率分别为（6．68±1．70）％和（6．03±1．85）％，均显著高于CP组[（4．74±0．93）％，P〈0．053。3组仔鼠10周龄OGTT结果差异均无统计学意义（P〉0．05）；DP-1组基础胰岛素水平[（0．76±0．37）μg／L]低于CP组及DP-2组[（1．13±0．25）μg／L和（1．06±0．14）μg／L，P〈0．05]；DP-1组平均葡萄糖输注速率高于DP-2组[（4．93±1．11）U／（kg·h）与（3．72±1．07）U／（kg·h），P〈0．05]。结论宫内高血糖环境暴露下的子代大鼠出生后3～10周体重增长过快，出生后早期过度喂养会加速胰岛素敏感性的下降。
Objective To establish the animal model of gestational diabetes mellitus （GDM） to observe the long-term effect of maternal hyperglycemia and postnatal overfeeding on the metabolism of offsprings. Methods Streptozotocin （STZ, 50 mg/kg） was administered to Wistar rats intraperitoneally on the 5th day of gestation to induce animal GDM model. Offsprings were allocated into 3 groups： Group CP （n=8） were exposed to normal maternal hyperglycemic environment, group DP-1 （n=8） exposed to intrauterine hyperglycemia and every 8 pups breast-fed by one GMD mother and group DP-2 exposed to intrauterine hyperglycemia and every 4 pups breast-fed by one GDM mother （n = 8）. Weight gain between 3-10 weeks were recorded, OGTT at 10 weeks and hyperinsulinemia-euglycemia clamp technique was performed, and plasma insulin level was measured by ELISA at 11 weeks. Results Offsprings in DP-1 and DP-2 group had greater weight gain than group CP at 3-10 weeks after birth[（6.68±1.70） % and （6. 03±1. 85） % vs （4.74±0.93） %, P〈 0. 05] but no difference was found among the three groups in OGTT results （P〉 0.05）. The baseline plasma insulin level was lower in the DP-1 group than in the DP-2 and CP group [（0.76±0.37）μg/L vs （1.13±0.25）μg/L and （1.06±0.14）μg/L]（P〈0.05）. GIR60-120 was higher in DP-1 than in DP-2 group [（4.93±1. 11）U/（kg·h） vs （3.72±1.07） U/（kg· h） ,P〈0.05]. Conclusions Offsprings of Wistar rats, exposed to intrauterine hyperglycemia, experience excessive weight gain at 3-10 weeks after birth. Overfeeding in early life expedites the decrease of insulin sensitivity in these offspings.
出处
《中华围产医学杂志》
CAS
2009年第6期430-433,共4页
Chinese Journal of Perinatal Medicine
关键词
糖尿病
实验性
糖尿病
妊娠
大鼠
动物
新生
体重增长
Diabetes mellitus, experimental
Diabetes, gestational
Rats
Animals, newborn
Weight gain
