摘要
目的探讨孕期重度高血糖及出生后早期过度喂养与子鼠成年期代谢表型的关系。方法成年Wistar雌性大鼠于妊娠第5大腹腔内注射20％链脲霉素50mg／kg，血糖〉20mmol／L为孕期重度高血糖模型（severely diabetes mellitus，SDM）。子鼠生后通过减少哺乳期胎仔数建立子鼠早期过度喂养模型。根据孕期血糖水平和哺乳期喂养方式将子鼠分为3组：（1）对照组子鼠（control pups，CP组）：孕期母鼠血糖正常，哺乳期1只母鼠喂养8只子鼠；（2）SDM正常喂养组：孕期母鼠重度高血糖，哺乳期1只母鼠喂养8只子鼠；（3）SDM过度喂养组：孕期母鼠重度高血糖，哺乳期1只母鼠喂养4只子鼠。各组子鼠于第3周断乳后均给予常规饲料喂养。观察3～26周各组子鼠体重变化，测定26周龄时子鼠收缩压、舒张压、心率以及代谢相关指标，包括空腹总甘油三酯、总胆固醇、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇，根据空腹血糖、空腹胰岛素水平利用胰岛素稳态模型（homeostasis model assessment，HOMA）计算胰岛素抵抗指数（insulin resistance，IR）。组间各指标比较采用ANOVA和LSD检验。结果SDM组母鼠空腹血糖为（28．34±5．14）mmol／L，明显高于对照组（6．25±1．41）mmol／L（P〈0．05）。SDM过度喂养组和正常喂养组子鼠3周断乳后体重显著低于CP组[（43．63±4．83）g、（31．45±10．21）g和（55．75±8．41）g，P〈0．05]，SDM过度喂养组3～6周体重均明显高于SDM正常喂养组（P〈0．05）。SDM组子鼠3～7周、3～9周体重增长率高于CP组，其中SDM正常喂养组与SDM过度喂养组和CP组间差异均有统计学意义（P〈0．05）。生后26周龄时SDM正常喂养组和SDM过度喂养组收缩压[（153．31±13．91）himHg和（147．21±12．29）mmHg]和甘油三酯[（0．73±0．22）mmol／L和（0．71±0．49）mmol／L]水平明显高于CP组[分别为（132．21±11．
Objective To explore the metabolic phenotype of the adult offspring rats with maternal severe hyperglycemia during pregnancy and overfeeding in early life. Methods The adult Wistar rats were administered intraperitoneally with 20% Streptozotocin （STZ, 50 rng/kg） on day 5 of pregnancy to induce severely gestational diabetes mellitus （SDM） model （blood glucose 20 mmol/L）. Early postnatal overfeeding models were established through reduction of the number of newborn rats fed by one mother rat. Offsprings were divided into 3 groups according to maternal blood glucose level during the pregnancy and feeding patterns during the lactation period （1） control pups （CP） ：maternal euglycemia was achieved during pregnancy and eight pups fed by one mother rat;（2） SDM-normal feeding group： SDM mothers and 8 pups fed by one mother rat; （3） SDM-overfeeding group：SDM mother and 4 pups fed by one mother rat. All pups were fed by standard laboratory chow adlibitum after weaning at 3 weeks of age. The body weight of all pups were measured weekly after weaning. At the age of 26 weeks, the systolic blood pressure （SBP）, diastolic blood pressure （DBP）, heart rate （HR） and metabolic markers, including fasting plasma glucose （FPG）, fasting insulin （FINS）, total triglyceride （TG）, total cholesterol （TC） , low density lipoprotein-cholestrol （LDL C） and high density lipoprotein-cholestrol （HDL C） were measured in all 3 groups. ANOVA and LSD test were applied in statistical analysis. Results The average plasma glucose level was significantly higher in the SDM mothers than in the controls [（28. 3±5.14） mmol/L vs （6.25±1.41） retool/L, P〈0. 05]. After weaning at 3 weeks, the weight of SDM-overfeeding group and SDM-normal feeding group was lighter than that of CP group [（43.63±4.83） g, （31.45±10.21） g vs （55.75±8.41） g, P〈0. 05], meanwhile, that of the SDM-overfeeding group were heavier than that of the SDM-normal feeding group （P〈 0.05）. Pups
出处
《中华围产医学杂志》
CAS
2010年第6期444-449,共6页
Chinese Journal of Perinatal Medicine
基金
国家自然科学基金（30973212）
关键词
妊娠并发症
高血糖症
母乳喂养
大鼠
代谢综合征X
Pregnancy complications
Hyperglycemia
Breast feeding
Rat
Metabolic syndrome X
