摘要
目的：建立蜂王浆及冻干粉中氯霉素残留的高效液相色谱-串联四极杆质谱联用测定方法。方法：该方法经乙酸乙酯提取样品,以Aglient SB-C18柱（2.1×150 mm,1.8μm）分离,流动相为水溶液和乙腈（体积比为35：65）,电喷雾负离子MRM模式检测。结果：该方法的检出限0.1 ng/ml,方法定量下限0.1 ng/kg,线性范围0.1～10.0 ng/ml,加标回收率87%～95%,相对标准偏差为3.21%。结论：该法具有样品预处理简单,灵敏度高,分析时间短等优点,易于推广。
Objective：To develop a LC-MS-MS method for chloramphenicol residues in royal jelly and royal jelly powder.Methods：The mass spectrometer was operated in the negative ion mode using multiple reaction monitoring.In the method,A sample was extracted with Ethyl acetate,Chromatographic separation was carried out on a Aglient SB-C18 column（2.1×150 mm,1.8 μm） with eluthic sion of acetonitrile-water（35：65 by volume）.Results：The limit of detection is 0.1 ng/ml and the limit of quantitation is 0.1 μg/kg,The linear plots were obtained between 0.1 ng/ml and 20.0 ng/ml.Overall recoveries were between 87%~95%,and RSD value was 3.2%.Conclusion：The advantages of this method are simple operation,shorter operation time and higher sensitivity.
出处
《中国卫生检验杂志》
CAS
2009年第12期2827-2828,共2页
Chinese Journal of Health Laboratory Technology
关键词
液质联用仪
氯霉素
蜂王浆及冻干粉
残留
LC/MS/ MS
Chloramphenicol
Royal jelly and royal jelly powder
Residue
