摘要
建立蜂蜜中氯霉素残留的高效液相色谱-串联四极杆质谱联用测定方法。该方法经乙酸乙酯提取样品,以ZORBAX EclipseXDB-C18柱(2.1mm×150mm,5μm)分离,流动相为水溶液-甲醇(40:60,V/V),电喷雾负离子MRM模式检测。该方法的检出限0.1ng/ml,样品的定量下限为0.1μg/kg,线性范围0.1～10.0ng/ml,加标回收率86.0%～93.2%,相对标准偏差为3.26%。
A LC-MS/MS method was developed for the detection of chloramphenicol residue in honey. Samples were extracted using ethyl acetate, and the extracts were separated on a ZORBAX XDB-C18 column （2.1 mm×150mm, 5 μ m） with a mobile phase composed of methanol and water （40：60, V/V）. The analysis was carried out by MS/MS in an electrospray negative ion multiple reaction monitoring （MRM） mode. The limits of detection and quantification were 0.1 ng/ml and 0.1 g/kg, respectively. The linear range was between 0.1 ng/ml and 10.0 ng/ml. The average spike recovery was 88.2%, with an average RSD of 3.26%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第14期285-287,共3页
Food Science
基金
农业部"948"项目(2006-G19)
农业部现代蜜蜂产业技术体系项目
