摘要
目的:观察多种营养素组合对链脲佐菌素(STZ)糖尿病小鼠血糖的影响,寻找有效改善1型糖尿病血糖控制的营养素组合.方法:以STZ140mg/kg诱导小鼠轻度糖尿病并随机分为对照组,糖尿病治疗1组(1组),糖尿病治疗2组(2组),糖尿病治疗3组(3组).另取正常小鼠作为正常对照组,每组20只.糖尿病治疗采用不同营养素复合制剂灌胃:1组以L-精氨酸盐酸盐+维生素A+尼克酰胺灌胃;2组以牛磺酸+维生素E+维生素C+尼克酰胺灌胃;3组以L-精氨酸盐酸盐+牛磺酸+生物素+尼克酰胺灌胃,每日早、晚各1次共4wk.营养素干预7d空腹8h,氧化酶法观察各组空腹血糖变化,于第28日进行糖耐量试验.28d空腹8h取胰腺,酸性乙醇提取胰岛素,间接放免法测定其浓度.Trizol法提取总RNA,经RT-PCR,电泳,凝胶成像分析系统成像并定量前胰岛素原mRNA水平.免疫组化染色以图像分析系统进行β细胞总量分析;WesternBlot法检测PDX-1蛋白表达.结果:营养素干预7d,空腹血糖1,2组小鼠未有显著改变,3组小鼠空腹血糖降至(6.65±1.34)mmol/L;在14,21,28d,糖尿病治疗3组空腹血糖分别为(6.32±1.36),(6.14±1.27),(6.25±1.43)mmol/L;糖耐量实验30,120min时间点葡萄糖曲线分别为(13.64±2.05),(6.26±2.46)mmol/L,各时间点数值与对照组相比较差异显著.糖尿病治疗3组胰腺组织胰岛素浓度(1.52±0.22)mU/L,显著高于对照组(0.42±0.11)mU/L;前胰岛素原mRNA表达水平(0.80±0.03),显著高于对照组(0.32±0.02);β细胞总量(5.11±1.56)mg与对照组(3.45±1.12)mg相比较差异显著(P<0.05);PDX-1蛋白表达也较对照组增加.结论:L-精氨酸盐酸盐+牛磺酸+生物素+尼克酰胺组合通过增加β细胞总量及胰岛素合成使STZ糖尿病小鼠空腹血糖及其糖耐量趋于正常.
AIM：To observe the effect of different nutrition combinations on blood glucose in STZ diabetic mice and to find the combination which can effectively control blood glucose in type 1 diabetes.METHODS：Mild diabetes mellitus were induced in male KM mice by a single intraperitoneal injection of STZ at a dose of 140 mg/kg.Diabetic mice were assigned randomly to the control group,three treatment groups（group 1,2 and 3）and normal mice were used as the normal group（n=20 in each group）.Group 1,2 and 3 were orally administered,respectively,with L-arginine hydrochloride＋vitamin A＋nicotinamide,taurine＋vitamin E＋vitaminC＋nicotinamide,L-arginine hydrochloride＋taurine＋biotin＋nicotinamide twice daily for 4 weeks.Fasting blood glucose in various groups were measured by an oxidase assay method at 7 th day of nutrition interference.Intraperitoneal glucose tolerance test was performed in the group where their blood glucose were significantly reduced at 28 th day of nutrition interference.The pancreas was removed at 28 th day.Insulun was extracted from pancreas by acid alcohol,and were determined by radioimmunoassay method.Total RNA was extracted from the pancreas by Trizol method.Preproinsulin gene was amplified by RT-PCR.The product was electrophoresed and quantified by image acquisition and analysis system.β cell mass was measured from the insulin antibody-stained sections using an image analysis system.PDX-1 pritein expression in the pancreas were detected by Western blotting.RESULTS：Fasting blood glucose in group 1 and group 2 were no significant change,and those in group 3 significantly decreased to（6.65±1.34）mmol/L at 7 th day of nutrition interference.The fasting glucose contents in group 3 were respectively（6.32±1.36）,（6.14±1.27）,（6.25±1.43）mmol/L at 14,21 and 28 thday.The glucose tolerance test shown that the values at points of time of 30 and 120 min in glucose curve were respectively（13.64±2.05）,（6.26±2.46）mmol/L,which were significantly different from those in
出处
《第四军医大学学报》
北大核心
2009年第22期2557-2560,共4页
Journal of the Fourth Military Medical University
基金
河北省科学技术研究与发展计划项目(07276101D-42)
关键词
营养素
糖尿病
小鼠
血糖
胰岛功能
nutrients diabetes mice blood glucose islet function
