摘要
目的:探讨姜黄素作用于新生大鼠坏死性小肠结肠炎(NEC)模型的疗效、抗炎作用及其机制.方法:经人工喂养、缺氧复氧冷冻及脂多糖灌胃后建立NEC动物模型.实验分为正常对照组(A组)、NEC模型组(B组)、姜黄素干预组(C组)及溶剂组(D组).各组于第4日处死大鼠,取近回盲部肠组织做病理切片,采用HE染色观察组织学变化并做组织损伤评分;免疫组化法检测NF-κB的表达.其他肠组织制备组织匀浆取上清用ELISA法检测肿瘤坏死因子α(TNF-α)和白细胞介素-6(IL-6)的含量.结果:A,D组大鼠无NEC症状,B组大鼠出现典型的NEC症状,C组大鼠与B组相比NEC症状出现的较晚、较轻.B组与A,D组相比肠组织病理学评分及NF-κB,TNF-α,IL-6的表达显著增高(P<0.01);C组与B组相比表达显著降低(P<0.05).结论:姜黄素对NEC大鼠具有保护作用,其机制可能是通过抑制NF-κB,TNF-α及IL-6的表达从而发挥抗炎效应.
AIM：To discuss the effect of curcumin and its anti-inflammatorymechanisms in necrotizing enterocolitis rat model.METHODS：NEC model of rats were established by artificialfeeding,cold exposure after hypoxic-reoxygenation treatment and LPS intragastric administration.The rats were randomly divided into normal control（group A）,model group（group B）,curcumine group（group C）and solvent group（group D）.All the subjects were put to death on the 4th day and proximal intestines of ileocecal junction were undertaken pathological sectioning.HE dying was carried out to observe histological changes and make a score of tissue damage.The expression of NF-κB was detected by immunohistochemical method.Other intestinal tissue was treated into homogenate.After the homogenate was centrifuged,supernates were tested the density of TNF-α and IL-6 with enzyme linked immunosorbent assay.RESULTS：There were not NEC symptoms in rats of groups A and D.The rats of group B appeared typical NEC symptoms.Compared with group B,the NEC symptoms of group C appeared later and lighter.The histopathologic scores and expression of NF-κB,TNF-α,IL-6 in group B significantly increased（P〈0.01）compared with groups A and D and group C significantly reduced（P〈0.05）compared with group B.CONCLUSION：Curcumin has a protective effect on rats of NEC.Its mechanism the restraint in NF-κB,TNF-α and IL-6 expressionsso as to have an anti-inflammation effect.
出处
《第四军医大学学报》
北大核心
2009年第22期2537-2540,共4页
Journal of the Fourth Military Medical University
基金
重庆市自然科学基金(CSCT
2007BB5326)
